PUBLICATION
Essential role of spi-1-like (spi-1l) in zebrafish myeloid cell differentiation
- Authors
- Bukrinsky, A., Griffin, K.J., Zhao, Y., Lin, S., and Banerjee, U.
- ID
- ZDB-PUB-090112-17
- Date
- 2009
- Source
- Blood 113(9): 2038-2046 (Journal)
- Registered Authors
- Bukrinsky, Alex, Griffin, Kevin, Lin, Shuo, Zhao, Yan
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Animals, Genetically Modified
- Cell Differentiation/genetics*
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental/drug effects
- Molecular Sequence Data
- Morpholines/chemistry
- Morpholines/pharmacology
- Myeloid Cells/metabolism
- Myeloid Cells/physiology*
- Oligonucleotides, Antisense/pharmacology
- Phylogeny
- Protein Structure, Tertiary
- Proto-Oncogene Proteins/chemistry
- Proto-Oncogene Proteins/genetics
- Proto-Oncogene Proteins/physiology
- Sequence Homology, Amino Acid
- Trans-Activators/chemistry
- Trans-Activators/genetics
- Trans-Activators/physiology
- Transcription Factors/chemistry
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Transcription Factors/physiology*
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- PubMed
- 19131555 Full text @ Blood
Citation
Bukrinsky, A., Griffin, K.J., Zhao, Y., Lin, S., and Banerjee, U. (2009) Essential role of spi-1-like (spi-1l) in zebrafish myeloid cell differentiation. Blood. 113(9):2038-2046.
Abstract
The ETS protein Spi-1/Pu.1 plays a pivotal and widespread role throughout hematopoiesis in many species. This study describes the identification, characterization and functional analysis of a new zebrafish spi transcription factor spi-1-like (spi-1l) that is expressed in primitive myeloid cells, erythro-myelo progenitor cells, and in the adult kidney. Spi-1l functions genetically downstream of etsrp, scl, and spi-1/pu.1 in myeloid differentiation. Spi-1l is co-expressed in a subset of spi-1/pu.1 cells and its function is necessary and sufficient for macrophage and granulocyte differentiation. These results establish a critical role for spi-1l in zebrafish myeloid cell differentiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping