PUBLICATION

A novel role for the endocannabinoid system during zebrafish development

Authors

Migliarini, B., and Carnevali, O.

ID

ZDB-PUB-081218-14

Date

2009

Source

Molecular and Cellular Endocrinology 299(2): 172-177 (Journal)

Registered Authors

Carnevali, Oliana

Keywords

CB1, AM251, zebrafish, oocytes, development

MeSH Terms

Oogenesis/drug effects
RNA, Messenger/genetics
RNA, Messenger/metabolism
Pyrazoles/pharmacology
Cannabinoid Receptor Modulators/metabolism*
Animals
Ovary/drug effects
Ovary/metabolism
Receptor, Cannabinoid, CB1/genetics
Receptor, Cannabinoid, CB1/metabolism
Locomotion/drug effects
Endocannabinoids*
Embryonic Development/drug effects
Zebrafish/embryology*
Zebrafish/genetics
Piperidines/pharmacology
Gene Expression Regulation, Developmental/drug effects
Embryo, Nonmammalian/drug effects
Embryo, Nonmammalian/metabolism
Male
RNA Transport/drug effects
Female

(all 22)

PubMed

19071191 Full text @ Mol. Cell. Endocrinol.

Abstract

The aim of this study was to increase our understanding of the physiological functions controlled by the endocannabinoid system during embryogenesis. Using genomic and proteomic methodologies applied to zebrafish, we proved, for the first time in an oviparous species, that the cannabinoid receptor CB1 is not a maternal factor. The analysis of different developmental stages showed that the zygotic expression of CB1 occurs from the 3 somites stage while CB1 protein becomes evident during hatching time, indicating an involvement in the hatching process. This result was supported by the data regarding embryo exposure to the CB1 antagonist, AM251, consisting in a 75% decrease in hatching rate. In addition, as previously described for mammals, we observed a role of CB1 in the motility behavior in zebrafish larvae.

Genes / Markers

Figures