PUBLICATION

Nogo-Nogo receptor signalling in PNS axon outgrowth and pathfinding

Authors

Brösamle, C., and Halpern, M.E.

ID

ZDB-PUB-081203-27

Date

2009

Source

Molecular and cellular neurosciences 40(4): 401-409 (Journal)

Registered Authors

Brösamle, Christian, Halpern, Marnie E.

Keywords

none

MeSH Terms

Alternative Splicing
Cell Movement/physiology*
Myelin Proteins/genetics
Myelin Proteins/metabolism*
Peripheral Nervous System*/anatomy & histology
Peripheral Nervous System*/embryology
Gene Expression Regulation, Developmental
Oligonucleotides, Antisense/genetics
Oligonucleotides, Antisense/metabolism
Signal Transduction/physiology*
Axons/metabolism*
Cranial Nerves/anatomy & histology
Cranial Nerves/embryology
Receptors, Cell Surface/genetics
Receptors, Cell Surface/metabolism*
Animals
Zebrafish*/anatomy & histology
Zebrafish*/embryology
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Protein Isoforms/genetics
Protein Isoforms/metabolism
Humans

(all 23)

PubMed

19041397 Full text @ Mol. Cell Neurosci.

Abstract

The Nogo/Nogo66 receptor signaling pathway has been characterized as inhibitory for axon growth, regeneration, and structural plasticity in the adult mammalian central nervous system. Nogo and its receptor are highly expressed when axon growth is abundant, however, the function of this pathway in neural development is unclear. We have characterized zebrafish Nogo pathway members and examined their role in the developing nervous system using anti-sense morpholinos that inhibit protein synthesis. Depletion of the Nogo66 receptor or a Nogo isoform causes truncated outgrowth of peripheral nervous system (PNS) axons of the head and lateral line. PNS nerves also show increased defasciculation and numerous guidance defects, including axons invading regions along the body flank that are normally avoided. We propose that localized Nogo expression defines inhibitory territories that through repulsion restrict axon growth to permissive regions.

Genes / Markers

Figures

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Expression

Fish	Conditions	Stage	Anatomy	Assay	Figure
WT	standard conditions	Long-pec	cranial ganglion	ISH	Fig. 2
WT	standard conditions	Long-pec	dorsal root ganglion	ISH	Fig. 2
WT	standard conditions	Long-pec	forebrain	ISH	Fig. 2
WT	standard conditions	Long-pec	lateral line ganglion	ISH	Fig. 2
WT	standard conditions	Long-pec	retina	ISH	Fig. 2
WT	standard conditions	Long-pec	Rohon-Beard neuron	ISH	Fig. 2
WT	standard conditions	Long-pec	vagal lobe	ISH	Fig. 2
WT	standard conditions	Prim-5	brain	ISH	Fig. 2
WT	standard conditions	Protruding-mouth	ciliary marginal zone	ISH	Fig. 2
WT	standard conditions	Long-pec	ciliary marginal zone	ISH	Fig. 2

1 - 10 of 34

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-rtn4r	standard conditions	Long-pec	axon extension disrupted, abnormal	Fig. 4
WT + MO1-rtn4r	standard conditions	Long-pec	axon guidance disrupted, abnormal	Fig. 4
WT + MO1-rtn4r	standard conditions	Long-pec	branching morphogenesis of a nerve disrupted, abnormal	Fig. 4
WT + MO1-rtn4r	standard conditions	Long-pec	peripheral nervous system neuron axonogenesis disrupted, abnormal	Fig. 4
WT + MO1-rtn4r	standard conditions	Long-pec	posterior lateral line nerve decreased length, abnormal	Fig. 4
WT + MO1-rtn4r	standard conditions	Long-pec	posterior lateral line nerve defasciculated, abnormal	Fig. 4
WT + MO1-rtn4r	standard conditions	Long-pec to Day 4	axon extension disrupted, abnormal	Fig. 4
WT + MO1-rtn4r	standard conditions	Long-pec to Day 4	posterior lateral line nerve decreased length, abnormal	Fig. 4
WT + MO1-rtn4r	standard conditions	Protruding-mouth	axon extension disrupted, abnormal	Fig. 3
WT + MO1-rtn4r	standard conditions	Protruding-mouth	axon guidance disrupted, abnormal	Fig. 3

1 - 10 of 38

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Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
rtn4a	MO1-rtn4a	MRPHLNO
rtn4a	MO2-rtn4a	MRPHLNO
rtn4a	MO3-rtn4a	MRPHLNO
rtn4r	MO1-rtn4r	MRPHLNO
rtn4r	MO2-rtn4r	MRPHLNO

Fish

Fish
WT
WT + MO1-rtn4r
WT + MO1-rtn4r + MO3-rtn4a
WT + MO2-rtn4r
WT + MO3-rtn4a

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Brösamle et al., 2009 ZDB-PUB-081203-27 PMID:19041397

Nogo-Nogo receptor signalling in PNS axon outgrowth and pathfinding

Brösamle et al., 2009

ZDB-PUB-081203-27
PMID:19041397