PUBLICATION
Regulation of zebrafish fin regeneration by microRNAs
- Authors
- Thatcher, E.J., Paydar, I., Anderson, K.K., and Patton, J.G.
- ID
- ZDB-PUB-081121-22
- Date
- 2008
- Source
- Proceedings of the National Academy of Sciences of the United States of America 105(47): 18384-18389 (Journal)
- Registered Authors
- Patton, James G., Thatcher, Elizabeth
- Keywords
- lef1, miR-203
- MeSH Terms
-
- 3' Untranslated Regions
- Animals
- Base Sequence
- Blotting, Northern
- Blotting, Western
- DNA Primers
- Fluorescent Antibody Technique
- MicroRNAs/genetics
- MicroRNAs/physiology*
- Regeneration/genetics*
- Reverse Transcriptase Polymerase Chain Reaction
- Transcription Factors/genetics
- Transcription Factors/physiology
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology
- PubMed
- 19015519 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Thatcher, E.J., Paydar, I., Anderson, K.K., and Patton, J.G. (2008) Regulation of zebrafish fin regeneration by microRNAs. Proceedings of the National Academy of Sciences of the United States of America. 105(47):18384-18389.
Abstract
A number of genes have been implicated in regeneration, but the regulation of these genes, particularly pertaining to regeneration in higher vertebrates, remains an interesting and mostly open question. We have studied microRNA (miRNA) regulation of regeneration and found that an intact miRNA pathway is essential for caudal fin regeneration in zebrafish. We also showed that miR-203 directly targets the Wnt signaling transcription factor Lef1 during this process. Repression of Lef1 by miR-203 blocks regeneration, whereas loss of miR-203 results in excess Lef1 levels and fin overgrowth. Expression of Lef1 from mRNAs lacking 3' UTR recognition elements can rescue the effects of excess miR-203, demonstrating that these effects are due to specific regulation of lef1 by miR-203. Our data support a model in which regulation of Lef1 protein levels by miR-203 is a key limiting step during regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping