ZFIN ID: ZDB-PUB-081121-13
Alpha2 macroglobulin-like is essential for liver development in zebrafish
Hong, S.K., and Dawid, I.B.
Date: 2008
Source: PLoS One   3(11): e3736 (Journal)
Registered Authors: Dawid, Igor B., Hong, Sung-Kook
Keywords: Embryos, Zebrafish, Pancreas, Untranslated regions, Gastrointestinal tract, Hematoxylin staining, In situ hybridization, Xenopus
MeSH Terms:
  • Animals
  • Cell Proliferation
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Gastrointestinal Tract/embryology
  • Gastrointestinal Tract/metabolism
  • Gene Expression Regulation, Developmental
  • Hepatocytes/cytology
  • In Situ Hybridization
  • In Situ Nick-End Labeling
  • Liver/cytology
  • Liver/embryology*
  • Liver/metabolism*
  • Oligonucleotides, Antisense/pharmacology
  • Organ Specificity
  • Organogenesis
  • Pancreas/embryology
  • Pancreas/metabolism
  • Phenotype
  • Phylogeny
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • alpha-Macroglobulins/genetics*
  • alpha-Macroglobulins/metabolism
PubMed: 19011686 Full text @ PLoS One
BACKGROUND: Alpha 2 Macroglobulin family members have been studied extensively with respect to their roles in physiology and human disease including innate immunity and Alzheimer's disease, but little is known about a possible role in liver development loss-of-function in model systems.
PRINCIPAL FINDINGS: We report the isolation of the zebrafish alpha2 macroglobulin-like (A2ML) gene and its specific expression in the liver during differentiation. Morpholino-based knock-down of A2ML did not block the initial formation of the liver primordium, but inhibited liver growth and differentiation.
SIGNIFICANCE: This report on A2ML function in zebrafish development provides the first evidence for a specific role of an A2M family gene in liver formation during early embryogenesis in a vertebrate.