|ZFIN ID: ZDB-PUB-081008-11|
Zili is required for germ cell differentiation and meiosis in zebrafish
Houwing, S., Berezikov, E., and Ketting, R.F.
|Source:||The EMBO journal 27(20): 2702-2711 (Journal)|
|Registered Authors:||Houwing, Saskia, Ketting, René|
|Keywords:||germ cell differentiation, meiosis, piRNAs, Piwi, zebrafish|
|PubMed:||18833190 Full text @ EMBO J.|
Houwing, S., Berezikov, E., and Ketting, R.F. (2008) Zili is required for germ cell differentiation and meiosis in zebrafish. The EMBO journal. 27(20):2702-2711.
ABSTRACTSmall RNAs exert an effect through diverse RNA interference pathways to transcriptionally or post-transcriptionally silence their targets. The Piwi-interacting RNAs (piRNAs) represent a germline-specific small RNA pathway where Piwi proteins themselves are thought to mediate piRNA biosynthesis. Here, we provide strong evidence for a piRNA amplification loop in zebrafish, in which Ziwi and Zili bind piRNAs of opposite polarity. Furthermore, we describe a function for Zili in transposon defense and germ cell differentiation, as well as a crucial function in meiosis, significantly extending the function of Piwi proteins beyond the control of transposable elements in vertebrates.