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ZFIN ID: ZDB-PUB-081008-11
Zili is required for germ cell differentiation and meiosis in zebrafish
Houwing, S., Berezikov, E., and Ketting, R.F.
Date: 2008
Source: The EMBO journal   27(20): 2702-2711 (Journal)
Registered Authors: Houwing, Saskia, Ketting, René
Keywords: germ cell differentiation, meiosis, piRNAs, Piwi, zebrafish
Microarrays: GEO:GSE12547
MeSH Terms:
  • Alleles
  • Animals
  • Cell Differentiation
  • Cell Nucleus/metabolism
  • Cloning, Molecular
  • Cytoplasm/metabolism
  • DNA Transposable Elements
  • Germ Cells/cytology*
  • Meiosis*
  • Models, Biological
  • Models, Genetic
  • RNA, Small Interfering/metabolism*
  • RNA-Binding Proteins/genetics
  • RNA-Binding Proteins/physiology*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed: 18833190 Full text @ EMBO J.
Small RNAs exert an effect through diverse RNA interference pathways to transcriptionally or post-transcriptionally silence their targets. The Piwi-interacting RNAs (piRNAs) represent a germline-specific small RNA pathway where Piwi proteins themselves are thought to mediate piRNA biosynthesis. Here, we provide strong evidence for a piRNA amplification loop in zebrafish, in which Ziwi and Zili bind piRNAs of opposite polarity. Furthermore, we describe a function for Zili in transposon defense and germ cell differentiation, as well as a crucial function in meiosis, significantly extending the function of Piwi proteins beyond the control of transposable elements in vertebrates.