ZFIN ID: ZDB-PUB-080908-14
Zebrafish and Frog Models of Mycobacterium marinum Infection
Cosma, C.L., Swaim, L.E., Volkman, H., Ramakrishnan, L., and Davis, J.M.
Date: 2006
Source: Current protocols in microbiology   Chapter 10: Unit10B.2 (Chapter)
Registered Authors: Cosma, Christine, Davis, James M., Ramakrishnan, Lalita, Swaim, Laura E., Volkman, Hannah
Keywords: Mycobacterium, animal models, zebrafish, fluorescence microscopy, infection
MeSH Terms:
  • Animals
  • Disease Models, Animal*
  • Embryo, Nonmammalian/microbiology
  • Host-Pathogen Interactions
  • Humans
  • Mycobacterium Infections, Nontuberculous/immunology
  • Mycobacterium Infections, Nontuberculous/microbiology
  • Mycobacterium Infections, Nontuberculous/mortality*
  • Mycobacterium Infections, Nontuberculous/pathology*
  • Mycobacterium marinum/isolation & purification
  • Mycobacterium marinum/pathogenicity*
  • Rana pipiens/microbiology*
  • Zebrafish/embryology
  • Zebrafish/microbiology*
PubMed: 18770575 Full text @ Curr. Protoc. Microbiol.
ABSTRACT
Mycobacterium marinum infection of poikilothermic animals, such as fish and frogs, results in chronic granulomatous diseases that bear many similarities to mycobacterioses in mammals, including tuberculosis. This unit describes three animal models of M. marinum infection that can be used to study basic aspects of Mycobacterium-host interactions and granuloma development, as well as trafficking of immune cells in host tissues. Protocols are included that describe intraperitoneal infection of adult leopard frogs (Rana pipiens) and zebrafish (Danio rerio). Protocols also describe subsequent monitoring of the infection by enumeration of bacterial cfu, mean time to death, or visual examination of infected tissue using both conventional histological stains and fluorescence microscopy of fluorescently marked bacteria. Furthermore, protocols are included that describe the infection of embryonic zebrafish and the subsequent analysis of the infection in real time using DIC and fluorescence microscopy.
ADDITIONAL INFORMATION No data available