PUBLICATION
MOZ/TIF2-induced acute myeloid leukaemia in transgenic fish
- Authors
- Zhuravleva, J., Paggetti, J., Martin, L., Hammann, A., Solary, E., Bastie, J.N., and Delva, L.
- ID
- ZDB-PUB-080902-17
- Date
- 2008
- Source
- British journal of haematology 143(3): 378-382 (Journal)
- Registered Authors
- Keywords
- MYST3/NCOA2, histone acetyl-transferase, acute myeloid leukaemia, zebrafish, animal model
- MeSH Terms
-
- Microinjections/methods
- Reverse Transcriptase Polymerase Chain Reaction/methods
- Animals, Genetically Modified
- Oncogene Proteins, Fusion/genetics*
- Oncogene Proteins, Fusion/metabolism
- Nuclear Receptor Coactivator 2/genetics*
- Nuclear Receptor Coactivator 2/metabolism
- Leukemia, Myeloid, Acute/genetics*
- Leukemia, Myeloid, Acute/metabolism
- Leukemia, Myeloid, Acute/pathology
- Zebrafish/genetics
- Disease Models, Animal
- Histone Acetyltransferases/genetics*
- Histone Acetyltransferases/metabolism
- Animals
- Gene Fusion
- Kidney/pathology
- PubMed
- 18729850 Full text @ Br. J. Haematol.
Citation
Zhuravleva, J., Paggetti, J., Martin, L., Hammann, A., Solary, E., Bastie, J.N., and Delva, L. (2008) MOZ/TIF2-induced acute myeloid leukaemia in transgenic fish. British journal of haematology. 143(3):378-382.
Abstract
The inv(8)(p11q13) chromosomal abnormality, described in acute myeloid leukaemias (AML), fuses the histone acetyl-transferase (HAT) MYST3 (MOZ) gene with another HAT gene, NCOA2 (TIF2). We generated a transgenic zebrafish in which the MYST3/NCOA2 fusion gene was expressed under control of the spi1 promoter. An AML developed in 2 of 180 MYST3/NCOA2-EGFP-expressing embryos, 14 and 26 months after injection of the fusion gene in a one-cell embryo, respectively. This leukaemia was characterised by an extensive invasion of kidneys by myeloid blast cells. This model, which is the first zebrafish model of AML, demonstrates the oncogenic potency of MYST3/NCOA2 fusion gene.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping