PUBLICATION
Motor neuron regeneration in adult zebrafish
- Authors
- Reimer, M.M., Sörensen, I., Kuscha, V., Frank, R.E., Liu, C., Becker, C.G., and Becker, T.
- ID
- ZDB-PUB-080826-36
- Date
- 2008
- Source
- The Journal of neuroscience : the official journal of the Society for Neuroscience 28(34): 8510-8516 (Journal)
- Registered Authors
- Becker, Catherina G., Becker, Thomas, Frank, Rebecca E., Kuscha, Veronika, Reimer, Michell M.
- Keywords
- endogenous stem cells, radial glia, BrdU, PCNA, SV2, adult neurogenesis
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- Bromodeoxyuridine
- Cell Count
- Cell Differentiation
- Cell Lineage
- Cell Proliferation
- Green Fluorescent Proteins/genetics
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- LIM-Homeodomain Proteins
- Microscopy, Electron
- Motor Neurons*/pathology
- Nerve Regeneration*
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Neuroglia/metabolism
- Neuroglia/pathology
- Phenotype
- Recombinant Fusion Proteins/genetics
- Spinal Cord/metabolism
- Spinal Cord/pathology
- Spinal Cord Injuries/pathology
- Spinal Cord Injuries/physiopathology*
- Stem Cells/metabolism
- Stem Cells/pathology
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Zebrafish*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 18716209 Full text @ J. Neurosci.
Citation
Reimer, M.M., Sörensen, I., Kuscha, V., Frank, R.E., Liu, C., Becker, C.G., and Becker, T. (2008) Motor neuron regeneration in adult zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 28(34):8510-8516.
Abstract
The mammalian spinal cord does not regenerate motor neurons that are lost as a result of injury or disease. Here we demonstrate that adult zebrafish, which show functional spinal cord regeneration, are capable of motor neuron regeneration. After a spinal lesion, the ventricular zone shows a widespread increase in proliferation, including slowly proliferating olig2-positive (olig2+) ependymo-radial glial progenitor cells. Lineage tracing in olig2:green fluorescent protein transgenic fish indicates that these cells switch from a gliogenic phenotype to motor neuron production. Numbers of undifferentiated small HB9+ and islet-1+ motor neurons, which are double labeled with the proliferation marker 5-bromo-2-deoxyuridine (BrdU), are transiently strongly increased in the lesioned spinal cord. Large differentiated motor neurons, which are lost after a lesion, reappear at 6-8 weeks after lesion, and we detected ChAT+/BrdU+ motor neurons that were covered by contacts immunopositive for the synaptic marker SV2. These observations suggest that, after a lesion, plasticity of olig2+ progenitor cells may allow them to generate motor neurons, some of which exhibit markers for terminal differentiation and integration into the existing adult spinal circuitry.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping