PUBLICATION
2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) and benzo[a]pyrene (BaP) in the gill and hepatopancreas of rpsL transgenic zebrafish
- Authors
- Amanuma, K., Tone, S., Nagaya, M., Matsumoto, M., Watanabe, T., Totsuka, Y., Wakabayashi, K., and Aoki, Y.
- ID
- ZDB-PUB-080825-15
- Date
- 2008
- Source
- Mutation research 656(1-2): 36-43 (Journal)
- Registered Authors
- Amanuma, Kimiko, Aoki, Yasunobu
- Keywords
- none
- MeSH Terms
-
- Gills/drug effects*
- Benzo(a)pyrene/chemistry
- Benzo(a)pyrene/toxicity*
- Animals
- Mutagens/toxicity*
- Triazoles/chemistry
- Triazoles/toxicity*
- Zebrafish/physiology*
- Water Pollutants, Chemical/toxicity*
- Mutagenicity Tests
- Salmonella/drug effects
- Point Mutation
- Animals, Genetically Modified
- Hepatopancreas/drug effects*
- PubMed
- 18675378 Full text @ Mutat. Res.
Citation
Amanuma, K., Tone, S., Nagaya, M., Matsumoto, M., Watanabe, T., Totsuka, Y., Wakabayashi, K., and Aoki, Y. (2008) 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) and benzo[a]pyrene (BaP) in the gill and hepatopancreas of rpsL transgenic zebrafish. Mutation research. 656(1-2):36-43.
Abstract
We examined the in vivo mutagenicity of 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) and benzo[a]pyrene (BaP) by using transgenic (Tg) zebrafish carrying the mutational target gene rpsL. PBTA-6 is one of the PBTA-type compounds that were recently identified in highly mutagenic river water in Japan. BaP is a well-known contaminant that is frequently found in polluted water. Both compounds are potent mutagens, as determined by using the Ames test employing S9 mix and Salmonella. Adult rpsL Tg zebrafish were exposed to 0, 7, or 10mg/L PBTA-6 or 0, 1.5, and 3mg/L BaP for 96h in a water bath and the mutations in their gills and hepatopancreata were measured 2-4 weeks later. At 3 weeks after exposure, 3mg/L BaP significantly increased the rpsL mutant frequency (MF) in the gill and hepatopancreas by 5- and 2.3-fold, respectively, as compared to control fish. Sequence analysis showed that BaP mainly induced G:C to T:A and G:C to C:G transversions, which is consistent with the known mutagenic effects of BaP. In contrast, despite its extremely high mutagenic potency in Salmonella strains, PBTA-6 did not significantly increase the MF in the zebrafish gill or hepatopancreas. Although PBTA-6 is 300 times more mutagenic than BaP in the Ames test [T. Watanabe, H. Nukaya, Y. Terao, Y. Takahashi, A. Tada, T. Takamura, H. Sawanishi, T. Ohe, T. Hirayama, T. Sugimura, K. Wakabayashi, Synthesis of 2-phenylbenzotriazole-type mutagens, PBTA-5 and PBTA-6, and their detection in river water from Japan, Mutat. Res. 498 (2001) 107-115], calculation of the mutagenicity per mole of compound indicated that PBTA-6 was 33- and <3.7-fold less mutagenic in the zebrafish gill and hepatopancreas, respectively, than BaP.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping