PUBLICATION
Molecular cloning of CYP1A gene and its expression by benzo(a)pyrene from goldfish (Carassius auratus)
- Authors
- Oh, S.M., Ryu, B.T., Kim, H.R., Choi, K., and Chung, K.H.
- ID
- ZDB-PUB-080728-18
- Date
- 2009
- Source
- Environmental toxicology 24(3): 225-234 (Journal)
- Registered Authors
- Keywords
- goldfish, CYP1A, cDNA cloning, benzo(a)pyrene
- MeSH Terms
-
- Kidney/metabolism
- Molecular Sequence Data
- Sequence Analysis, DNA
- Cloning, Molecular
- Amino Acid Sequence
- Bile/metabolism
- Cytochrome P-450 CYP1A1/classification
- Cytochrome P-450 CYP1A1/genetics*
- Cytochrome P-450 CYP1A1/metabolism
- Animals
- Base Sequence
- Sequence Alignment
- Benzo(a)pyrene/pharmacokinetics
- Benzo(a)pyrene/pharmacology*
- Gene Expression Regulation, Enzymologic/drug effects*
- Goldfish/genetics*
- Goldfish/metabolism
- Liver/metabolism
- PubMed
- 18655176 Full text @ Env. Tox.
Citation
Oh, S.M., Ryu, B.T., Kim, H.R., Choi, K., and Chung, K.H. (2009) Molecular cloning of CYP1A gene and its expression by benzo(a)pyrene from goldfish (Carassius auratus). Environmental toxicology. 24(3):225-234.
Abstract
We cloned and sequenced the cytochrome P450 1A (CYP1A) gene from goldfish (Carassius auratus). It has a 1581 bp open reading frame that encodes a 526 amino acid protein with a theoretical molecular weight of 59.02 kDa. The CYP1A amino acid sequence clusters in a monophyletic group with other fish CYP1As, and more closely related to zebrafish CYP1A (91% identity) than to other fish CYP1As. Exposure to benzo(a)pyrene (BaP) by intraperitoneal injection increased biliary BaP metabolites and liver CYP1A gene expression. BaP exposure also increased CYP1A gene expression in extrahepatic organs, including intestine, and gill, which are sensitive to aqueous and dietary exposure to Arylhydrocarbon receptor (AhR) agonists. Therefore, goldfish CYP1A identified in this study offers basic information for further research related to biomarker use of CYP1A of goldfish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping