PUBLICATION
Immune response of zebrafish (Danio rerio) against a newly isolated bacterial pathogen Aeromonas hydrophila
- Authors
- Rodríguez, I., Novoa, B., and Figueras, A.
- ID
- ZDB-PUB-080722-29
- Date
- 2008
- Source
- Fish & shellfish immunology 25(3): 239-249 (Journal)
- Registered Authors
- Figueras, Antonio, Novoa, Beatriz
- Keywords
- Zebrafish, Aeromonas, Cytokine, Inflammation, Immune response, Flow cytometry, Extracellular products
- MeSH Terms
-
- Aeromonas hydrophila/enzymology
- Aeromonas hydrophila/isolation & purification
- Aeromonas hydrophila/pathogenicity
- Aeromonas hydrophila/physiology*
- Animals
- Cell Survival
- Cells, Cultured
- Cytokines/genetics
- Flatfishes/immunology
- Flatfishes/microbiology
- Hemolysis/immunology
- Kidney/cytology
- Kidney/metabolism
- Kidney/pathology
- Lethal Dose 50
- Nitric Oxide/metabolism
- Phylogeny
- RNA, Ribosomal, 16S/genetics
- Reactive Oxygen Species/metabolism
- Zebrafish/immunology*
- Zebrafish/microbiology*
- PubMed
- 18640853 Full text @ Fish Shellfish Immunol.
Citation
Rodríguez, I., Novoa, B., and Figueras, A. (2008) Immune response of zebrafish (Danio rerio) against a newly isolated bacterial pathogen Aeromonas hydrophila. Fish & shellfish immunology. 25(3):239-249.
Abstract
A strain of Aeromonas hydrophila associated with unusual mortalities in zebrafish (Danio rerio) culture facilities was isolated, identified and characterized. In challenge experiments, adult zebrafish were susceptible to infection by intraperitoneal (i.p.) injection with viable bacteria and its extracellular products (ECPs) reaching very high mortalities in a few hours. The infection, by the viable bacteria or the ECPs, caused cell death in kidney, due to the cytotoxic and haemolytic activities of the bacterial ECPs. Moreover, the infection affected the release of oxygen (ROS) and nitrogen (NO) reactive free radicals. To determine if this A. hydrophila infection induces an inflammatory response, mRNA expression levels of tumour necrosis factor-alpha (TNFalpha), interleukin-1beta (IL-1beta), interferon-gamma (IFNgamma) and inducible nitric oxide (iNOS) were assessed by real time PCR. The expression levels of TNFalpha, IL-1beta and IFNgamma were upregulated in the kidneys of infected zebrafish with viable bacteria, heat-killed bacteria and ECPs. Expression levels of iNOS were upregulated by ECPs. Mortality rate (LD(50)) and histopathology were also determined.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping