PUBLICATION

Carboxypeptidase A5 identifies a novel mast cell lineage in the zebrafish providing new insight into mast cell fate determination

Authors
Dobson, J.T., Seibert, J., Teh, E.M., Daas, S., Fraser, R.B., Paw, B.H., Lin, T.J., and Berman, J.N.
ID
ZDB-PUB-080722-21
Date
2008
Source
Blood   112(7): 2969-2972 (Journal)
Registered Authors
Berman, Jason, Dobson, Tristan, Paw, Barry, Seibert, Jake
Keywords
none
MeSH Terms
  • Animals
  • Carboxypeptidases A/metabolism*
  • Cell Lineage*
  • Mast Cells/cytology*
  • Mast Cells/enzymology*
  • Stem Cells/cytology
  • Stem Cells/enzymology
  • Zebrafish/metabolism*
PubMed
18635811 Full text @ Blood
Abstract
Mast cells (MCs) play critical roles in allergy and inflammation, yet their development remains controversial due to limitations posed by traditional animal models. The zebrafish provides a highly efficient system for studying vertebrate hematopoiesis. We have identified zebrafish MCs in the gill and intestine, which resemble their mammalian counterparts both structurally and functionally. Carboxypeptidase A5 (cpa5), a MC specific enzyme, is expressed in zebrafish blood cells beginning at 24 hours post fertilization (hpf). At 28 hpf, co-localization is observed with pu.1, mpo, l-plastin and lysozyme C, but not fms or cepbalpha, identifying these early MCs as a distinct myeloid population arising from a common granulocyte/monocyte progenitor. Morpholino "knockdown" studies demonstrate transcription factors gata-2 and pu.1, but not gata-1 or fog-1 as necessary for early MC development. These studies validate the zebrafish as an in vivo tool for studying MC ontogeny and function, with future capacity for modeling human MC diseases.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes