PUBLICATION
Carboxypeptidase A5 identifies a novel mast cell lineage in the zebrafish providing new insight into mast cell fate determination
- Authors
- Dobson, J.T., Seibert, J., Teh, E.M., Daas, S., Fraser, R.B., Paw, B.H., Lin, T.J., and Berman, J.N.
- ID
- ZDB-PUB-080722-21
- Date
- 2008
- Source
- Blood 112(7): 2969-2972 (Journal)
- Registered Authors
- Berman, Jason, Dobson, Tristan, Paw, Barry, Seibert, Jake
- Keywords
- none
- MeSH Terms
-
- Animals
- Carboxypeptidases A/metabolism*
- Cell Lineage*
- Mast Cells/cytology*
- Mast Cells/enzymology*
- Stem Cells/cytology
- Stem Cells/enzymology
- Zebrafish/metabolism*
- PubMed
- 18635811 Full text @ Blood
Citation
Dobson, J.T., Seibert, J., Teh, E.M., Daas, S., Fraser, R.B., Paw, B.H., Lin, T.J., and Berman, J.N. (2008) Carboxypeptidase A5 identifies a novel mast cell lineage in the zebrafish providing new insight into mast cell fate determination. Blood. 112(7):2969-2972.
Abstract
Mast cells (MCs) play critical roles in allergy and inflammation, yet their development remains controversial due to limitations posed by traditional animal models. The zebrafish provides a highly efficient system for studying vertebrate hematopoiesis. We have identified zebrafish MCs in the gill and intestine, which resemble their mammalian counterparts both structurally and functionally. Carboxypeptidase A5 (cpa5), a MC specific enzyme, is expressed in zebrafish blood cells beginning at 24 hours post fertilization (hpf). At 28 hpf, co-localization is observed with pu.1, mpo, l-plastin and lysozyme C, but not fms or cepbalpha, identifying these early MCs as a distinct myeloid population arising from a common granulocyte/monocyte progenitor. Morpholino "knockdown" studies demonstrate transcription factors gata-2 and pu.1, but not gata-1 or fog-1 as necessary for early MC development. These studies validate the zebrafish as an in vivo tool for studying MC ontogeny and function, with future capacity for modeling human MC diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping