PUBLICATION
Zebrafish with mutations in mismatch repair genes develop neurofibromas and other tumors
- Authors
- Feitsma, H., Kuiper, R.V., Korving, J., Nijman, I.J., and Cuppen, E.
- ID
- ZDB-PUB-080707-5
- Date
- 2008
- Source
- Cancer research 68(13): 5059-5066 (Journal)
- Registered Authors
- Cuppen, Edwin, Feitsma, Harma
- Keywords
- zebrafish, mismatch repair, cancer, neurofibroma, microsatellite instability
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- DNA Repair Enzymes/genetics*
- DNA-Binding Proteins/genetics
- Male
- Neoplasms/genetics*
- Base Sequence
- Nerve Sheath Neoplasms/genetics
- Neurofibromatoses/genetics*
- Zebrafish/genetics*
- Microsatellite Instability
- Abdominal Neoplasms/genetics
- Brain Neoplasms/genetics
- DNA Mutational Analysis
- Mutation/physiology
- DNA Mismatch Repair*
- Female
- Hemangiosarcoma/genetics
- Amino Acid Sequence
- Eye Neoplasms/genetics
- MutS Homolog 2 Protein/genetics
- PubMed
- 18593904 Full text @ Cancer Res.
Citation
Feitsma, H., Kuiper, R.V., Korving, J., Nijman, I.J., and Cuppen, E. (2008) Zebrafish with mutations in mismatch repair genes develop neurofibromas and other tumors. Cancer research. 68(13):5059-5066.
Abstract
Defective mismatch repair (MMR) in humans causes hereditary nonpolyposis colorectal cancer. This genetic predisposition to colon cancer is linked to heterozygous familial mutations, and loss-of-heterozygosity is necessary for tumor development. In contrast, the rare cases with biallelic MMR mutations are juvenile patients with brain tumors, skin neurofibromas, and café-au-lait spots, resembling the neurofibromatosis syndrome. Many of them also display lymphomas and leukemias, which phenotypically resembles the frequent lymphoma development in mouse MMR knockouts. Here, we describe the identification and characterization of novel knockout mutants of the three major MMR genes, mlh1, msh2, and msh6, in zebrafish and show that they develop tumors at low frequencies. Predominantly, neurofibromas/malignant peripheral nerve sheath tumors were observed; however, a range of other tumor types was also observed. Our findings indicate that zebrafish mimic distinct features of the human disease and are complementary to mouse models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping