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ZFIN ID: ZDB-PUB-080701-5
Spatio-temporal expression of Chromogranin A during zebrafish embryogenesis
Xie, J., Wang, W., Liu, T., Deng, M., and Ning, G.
Date: 2008
Source: The Journal of endocrinology   198(3): 451-458 (Journal)
Registered Authors: Deng, Min, Liu, Ting Xi, Xie, Jing
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Chromogranin A/chemistry
  • Chromogranin A/classification
  • Chromogranin A/genetics
  • Chromogranin A/metabolism*
  • Computational Biology
  • Embryo, Nonmammalian/metabolism*
  • Gene Expression Regulation, Developmental*/physiology
  • In Situ Hybridization
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Homology, Amino Acid
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/classification
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 18586978 Full text @ J. Endocrinol.
FIGURES
ABSTRACT
Chromogranin A (CHGA), a protein participating in the biogenesis of dense core secretory granules in various neuroendocrine tissues, plays a critical role in the release of hormones/peptides and the pathogenesis of pheochromocytoma. However, little is known about the developmental origin of CHGA-expressing cells during embryogenesis. Here we report the structural characterization and spatio-temporal expression pattern of zebrafish (Danio rerio) orthologue of mammalian CHGA. The earliest expression of chga transcripts was observed at 16 hours post-fertilization (hpf) in the developing cranial ganglia as six distinct cellular masses arranged bilaterally as strings of beads in the dorsal root ganglia (DRG) precursors along the dorsal trunk. With development advancing, the chga transcripts were expressed abundantly in diencephalon, mesencephalon and rhombencephalon as well as in the DRG. Interestingly, double in situ hybridization assay of chga with genes expressed in pronephros (Wilms' tumor suppressor 1, wt1), adrenal cortex (side-chain cleavage-enzyme, scc) and sympathoadrenal neuron/chromaffin cell (dopamine-beta-hydroxylase, dbetah) respectively, showed that the chga-expressing cells are spatially separated from wt1-, scc-, and dbetah-positive cell populations during early embryonic development. The pronephros region does not express chga even up to 7 days post-fertilization (dpf), while chga positive-staining cells bound in the brain and DRG indicating that chga may play an important role in nervous system development during the early embryonic stages.
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