PUBLICATION
Expression of mt2 and smt-B upon cadmium exposure and cold shock in zebrafish (Danio rerio)
- Authors
- Wu, S.M., Zheng, Y.D., and Kuo, C.H.
- ID
- ZDB-PUB-080630-11
- Date
- 2008
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 148(2): 184-193 (Journal)
- Registered Authors
- Keywords
- Adaptation, Development, Isoform, Phylogenetic, Teleost, Transcripts
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cadmium Chloride/toxicity*
- Cold Temperature*
- Dose-Response Relationship, Drug
- Gene Expression Regulation, Developmental/drug effects
- In Situ Hybridization
- Larva/drug effects
- Larva/metabolism
- Metallothionein/genetics
- Metallothionein/metabolism*
- Molecular Sequence Data
- Phylogeny
- RNA, Messenger/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Time Factors
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 18579444 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Wu, S.M., Zheng, Y.D., and Kuo, C.H. (2008) Expression of mt2 and smt-B upon cadmium exposure and cold shock in zebrafish (Danio rerio). Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 148(2):184-193.
Abstract
Metallothionein-2 (mt2) and similar to metallothionein-B (smt-B) are included in the MT gene family. The objective of this study was to compare mt2 and smt-B messenger (m)RNA expressions after cadmium exposure and cold shock with whole-mount in situ hybridization in immature zebrafish (Danio rerio) and with a semi-quantitative RT-PCR in mature zebrafish. Three-day post-fertilization (dpf) larvae were treated with 0, 0.08, 0.26, and 0.89 muM cadmium for 24 and 48 h, and some larvae were challenged with a normal (28.5 degrees C) or low temperature (12 degrees C) for 12, 24, and 48 h. Results were obtained. (1) During embryonic and larval development, mt2 mRNA existed at 6 h post-fertilization (hpf), and the level rapidly increased to 24 hpf, then it gradually increased with further larval growth. smt-B was found at 12 hpf, and it also rapidly increased to 24 hpf, but remained constant during further larval development. (2) The mt2 mRNA signals and whole-body Cd contents displayed dose- and time-dependent responses after Cd exposure. After cold shock, mt2 mRNA signals also showed time-dependent expression. But smt-B mRNA signals were not appeared by either challenge. Besides, mature zebrafish were treated with 1.78 muM Cd and found that the highest levels of smt-B mRNA (smt-B/beta-actin) appeared in brain, and seems a reverse expression between smt-B mRNA and mt2 in brain after Cd exposure. Apparently, mt2 is possibly more relevant to Cd detoxification and cold shock adaptation in zebrafish larvae compared to smt-B, but smt-B might be related to certain physiological functions in neural (or brain) of mature zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping