PUBLICATION

Transient up-regulation of a novel member of Spot 14 family in androgen-stimulated rat prostate

Authors
Nishi, N., Shoji, H., Miyanaka, H., and Nakamura, T.
ID
ZDB-PUB-080602-6
Date
2008
Source
Biochimica et biophysica acta. General subjects   1780(7-8): 1004-1009 (Journal)
Registered Authors
Keywords
Prostate, Androgen, cDNA subtraction, Spot 14, Mig12
MeSH Terms
  • Dimerization
  • Proto-Oncogene Proteins pp60(c-src)/chemistry
  • Proto-Oncogene Proteins pp60(c-src)/genetics
  • Proto-Oncogene Proteins pp60(c-src)/metabolism*
  • Injections, Subcutaneous
  • Mutation
  • Prostate/drug effects*
  • Prostate/metabolism*
  • Sequence Homology, Amino Acid
  • Amino Acid Sequence
  • Animals
  • Adaptor Proteins, Signal Transducing/chemistry
  • Adaptor Proteins, Signal Transducing/genetics
  • Adaptor Proteins, Signal Transducing/metabolism*
  • Time Factors
  • Androgens/administration & dosage
  • Androgens/pharmacology*
  • Male
  • Molecular Weight
  • DNA, Complementary/genetics
  • Molecular Sequence Data
  • Orchiectomy
  • Rats
  • RNA, Messenger/chemistry
  • RNA, Messenger/metabolism
  • Testosterone Propionate/administration & dosage
  • Testosterone Propionate/pharmacology
  • Rats, Sprague-Dawley
  • Up-Regulation/drug effects*
(all 29)
PubMed
18505691 Full text @ BBA General Subjects
Abstract
The rat prostate is dependent on androgen for growth and differentiation. In an effort to find novel genes involved in androgen-induced growth of the rat prostate, we carried out PCR-based subtractive hybridization and identified several factors that were transiently up-regulated after androgen stimulation in castrated rat prostate. Among them, a novel member of the Spot 14 family has been identified. This protein (Spot 14-like androgen-inducible protein, SLAP) exhibited the highest homology (about 50%) with zebrafish gastrulation-specific G12 protein and about 30% homology with rat Spot 14. The SLAP mRNA level decreased following castration and transiently increased after testosterone replacement, attaining a peak 3 days after the treatment. The change in the protein level was similar to that in mRNA except that it was low in both untreated and castrated rat prostate tissue. In normal adult rats, SLAP was expressed at relatively high levels in the lung, stomach and liver. Unlike the prostate, SLAP expression in the lung was not affected by the androgen status. Like other members of the Spot 14 family, SLAP has a leucine-zipper motif in its C-terminal region, making it possible to form a stable homodimer. Though the physiological role of SLAP remains to be clarified, the current results suggest that SLAP expression is associated with some growth-related processes in regrowing rat prostate.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Nishi, N., Shoji, H., Miyanaka, H., and Nakamura, T. (2008) Transient up-regulation of a novel member of Spot 14 family in androgen-stimulated rat prostate. Biochimica et biophysica acta. General subjects. 1780(7-8):1004-1009.