PUBLICATION
Changes of phospho-growth-associated protein 43 (phospho-GAP43) in the zebrafish retina after optic nerve injury: A long-term observation
- Authors
- Kaneda, M., Nagashima, M., Nunome, T., Muramatsu, T., Yamada, Y., Kubo, M., Muramoto, K., Matsukawa, T., Koriyama, Y., Sugitani, K., Vachkov, I.H., Mawatari, K., and Kato, S.
- ID
- ZDB-PUB-080527-3
- Date
- 2008
- Source
- Neuroscience research 61(3): 281-288 (Journal)
- Registered Authors
- Nagashima, Mikiko
- Keywords
- Zebrafish, Optic nerve regeneration, Phospho-GAP43, Axon growth, Synaptic refinement, Chasing behavior
- MeSH Terms
-
- RNA, Messenger/metabolism
- Optic Nerve Injuries/metabolism
- Optic Nerve Injuries/pathology*
- Behavior, Animal
- Glial Fibrillary Acidic Protein/metabolism
- Retina/metabolism*
- GAP-43 Protein/genetics
- GAP-43 Protein/metabolism*
- Time Factors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Zebrafish/metabolism
- Gene Expression Regulation/physiology*
- Visual Pathways/metabolism
- Visual Pathways/pathology
- Animals
- Nerve Regeneration/physiology
- Analysis of Variance
- Axotomy/methods
- Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate/metabolism
- PubMed
- 18485507 Full text @ Neurosci. Res.
Citation
Kaneda, M., Nagashima, M., Nunome, T., Muramatsu, T., Yamada, Y., Kubo, M., Muramoto, K., Matsukawa, T., Koriyama, Y., Sugitani, K., Vachkov, I.H., Mawatari, K., and Kato, S. (2008) Changes of phospho-growth-associated protein 43 (phospho-GAP43) in the zebrafish retina after optic nerve injury: A long-term observation. Neuroscience research. 61(3):281-288.
Abstract
The major model animal of optic nerve regeneration in fish is goldfish. A closely related zebrafish is the most popular model system for genetic and developmental studies of vertebrate central nervous system. A few challenging works of optic nerve regeneration have been done with zebrafish. However, knowledge concerning the long term of optic nerve regeneration apparently lacks in zebrafish. In the present study, therefore, we followed changes of zebrafish behavior and phosphorylated form of growth-associated protein 43 (phospho-GAP43) expression in the zebrafish retina over 100 days after optic nerve transection. Optomotor response was fast recovered by 20-25 days after axotomy whereas chasing behavior (a schooling behavior) was slowly recovered by 80-100 days after axotomy. The temporal pattern of phospho-GAP43 expression showed a biphasic increase, a short-peak (12 folds) at 1-2 weeks and a long-plateau (4 folds) at 1-2 months after axotomy. The recovery of optomotor response well correlated with projection of growing axons to the tectum, whereas the recovery of chasing behavior well correlated with synaptic refinement of retinotectal topography. The present data strongly suggest that phospho-GAP43 plays an active role in both the early and late stages of optic nerve regeneration in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping