PUBLICATION
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
- Authors
- Doyon, Y., McCammon, J.M., Miller, J.C., Faraji, F., Ngo, C., Katibah, G.E., Amora, R., Hocking, T.D., Zhang, L., Rebar, E.J., Gregory, P.D., Urnov, F.D., and Amacher, S.L.
- ID
- ZDB-PUB-080527-24
- Date
- 2008
- Source
- Nat. Biotechnol. 26(6): 702-708 (Journal)
- Registered Authors
- Amacher, Sharon, McCammon, Jasmine
- Keywords
- none
- MeSH Terms
-
- Animals
- Deoxyribonucleases/genetics
- Mutagenesis, Site-Directed/methods*
- Zebrafish/genetics*
- Protein Engineering/methods
- Gene Targeting/methods*
- Zebrafish Proteins/genetics*
- Genetic Engineering/methods*
- Animals, Genetically Modified/physiology*
- Zinc Fingers/genetics*
- PubMed
- 18500334 Full text @ Nat. Biotechnol.
Citation
Doyon, Y., McCammon, J.M., Miller, J.C., Faraji, F., Ngo, C., Katibah, G.E., Amora, R., Hocking, T.D., Zhang, L., Rebar, E.J., Gregory, P.D., Urnov, F.D., and Amacher, S.L. (2008) Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nat. Biotechnol.. 26(6):702-708.
Abstract
We describe the use of zinc-finger nucleases (ZFNs) for somatic and germline disruption of genes in zebrafish (Danio rerio), in which targeted mutagenesis was previously intractable. ZFNs induce a targeted double-strand break in the genome that is repaired to generate small insertions and deletions. We designed ZFNs targeting the zebrafish golden and no tail/Brachyury (ntl) genes and developed a budding yeast-based assay to identify the most active ZFNs for use in vivo. Injection of ZFN-encoding mRNA into one-cell embryos yielded a high percentage of animals carrying distinct mutations at the ZFN-specified position and exhibiting expected loss-of-function phenotypes. Over half the ZFN mRNA-injected founder animals transmitted disrupted ntl alleles at frequencies averaging 20%. The frequency and precision of gene-disruption events observed suggest that this approach should be applicable to any loci in zebrafish or in other organisms that allow mRNA delivery into the fertilized egg.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping