ZFIN ID: ZDB-PUB-080527-13
RNAi suppression of zebrafish peptidoglycan recognition protein 6 (zfPGRP6) mediated differentially expressed genes involved in Toll-like receptor signaling pathway and caused increased susceptibility to Flavobacterium columnare
Chang, M.X., and Nie, P.
Date: 2008
Source: Veterinary Immunology and Immunopathology   124(3-4): 295-301 (Journal)
Registered Authors: Chang, Mingxian, Nie, P
Keywords: Zebrafish, Flavobacterium columnare, zfPGRP6, TLR, β-Defensin
MeSH Terms:
  • Animals
  • Carrier Proteins/antagonists & inhibitors
  • Carrier Proteins/genetics*
  • Carrier Proteins/immunology
  • DNA/chemistry
  • DNA/genetics
  • Flavobacteriaceae Infections/genetics
  • Flavobacteriaceae Infections/immunology
  • Flavobacteriaceae Infections/veterinary*
  • Flavobacteriaceae Infections/virology
  • Flavobacterium/immunology*
  • Gene Silencing
  • Interleukin-1alpha/chemistry
  • Interleukin-1alpha/genetics
  • Myeloid Differentiation Factor 88/chemistry
  • Myeloid Differentiation Factor 88/genetics
  • NF-kappa B/chemistry
  • NF-kappa B/genetics
  • Polymerase Chain Reaction/veterinary
  • RNA Interference
  • RNA, Small Interfering/genetics
  • Signal Transduction
  • Toll-Like Receptors/genetics
  • Toll-Like Receptors/immunology
  • Toll-Like Receptors/metabolism*
  • Zebrafish/genetics
  • Zebrafish/immunology*
  • beta-Defensins/chemistry
  • beta-Defensins/genetics
PubMed: 18495251 Full text @ Vet. Immunol. Immunopathol.
In Drosophila, Toll signaling cascade, which resembles the mammalian Toll-like receptor (TLR)/IL-1R signaling pathways and regulates the expression of anti-microbial peptide genes, mainly relies on peptidoglycan recognition proteins (PGRPs) for the detection of bacterial pathogens. To explore the effect of zebrafish peptidoglycan recognition protein 6 (zfPGRP6) on Toll-like receptor signaling pathway, RNA interference (siRNA) and real time quantitative PCR (RQ-PCR) methods were used to identify differentially expressed genes regulated by zfPGRP6. The target genes included TLR2, TLR3, TLR5, TLR7, TLR8, IL1R, Sterile-alpha and Armadillo motif containing protein (SARM), myeloid differentiation factor 88 (MyD88) and nuclear factor (NF)-kappa B2 (p100/p52). The results of RQ-PCR showed that RNAi-mediated suppression of zfPGRP6 significantly down-regulated the expression of TLR2, TLR5, IL1R, SARM, MyD88 and p100/p52. The expression of beta-defensin-1 was also down-regulated in those embryos silenced by zfPGRP6. In challenge experiments to determine the anti-bacterial response to Gram-negative bacteria, RNAi knock-down of zfPGRP6 markedly increased susceptibility to Flavobacterium columnare.