PUBLICATION
RNAi suppression of zebrafish peptidoglycan recognition protein 6 (zfPGRP6) mediated differentially expressed genes involved in Toll-like receptor signaling pathway and caused increased susceptibility to Flavobacterium columnare
- Authors
- Chang, M.X., and Nie, P.
- ID
- ZDB-PUB-080527-13
- Date
- 2008
- Source
- Veterinary Immunology and Immunopathology 124(3-4): 295-301 (Journal)
- Registered Authors
- Chang, Mingxian, Nie, Pin
- Keywords
- Zebrafish, Flavobacterium columnare, zfPGRP6, TLR, β-Defensin
- MeSH Terms
-
- Animals
- Carrier Proteins/antagonists & inhibitors
- Carrier Proteins/genetics*
- Carrier Proteins/immunology
- DNA/chemistry
- DNA/genetics
- Flavobacteriaceae Infections/genetics
- Flavobacteriaceae Infections/immunology
- Flavobacteriaceae Infections/veterinary*
- Flavobacteriaceae Infections/virology
- Flavobacterium/immunology*
- Gene Silencing
- Interleukin-1alpha/chemistry
- Interleukin-1alpha/genetics
- Myeloid Differentiation Factor 88/chemistry
- Myeloid Differentiation Factor 88/genetics
- NF-kappa B/chemistry
- NF-kappa B/genetics
- Polymerase Chain Reaction/veterinary
- RNA Interference
- RNA, Small Interfering/genetics
- Signal Transduction
- Toll-Like Receptors/genetics
- Toll-Like Receptors/immunology
- Toll-Like Receptors/metabolism*
- Zebrafish/genetics
- Zebrafish/immunology*
- beta-Defensins/chemistry
- beta-Defensins/genetics
- PubMed
- 18495251 Full text @ Vet. Immunol. Immunopathol.
Citation
Chang, M.X., and Nie, P. (2008) RNAi suppression of zebrafish peptidoglycan recognition protein 6 (zfPGRP6) mediated differentially expressed genes involved in Toll-like receptor signaling pathway and caused increased susceptibility to Flavobacterium columnare. Veterinary Immunology and Immunopathology. 124(3-4):295-301.
Abstract
In Drosophila, Toll signaling cascade, which resembles the mammalian Toll-like receptor (TLR)/IL-1R signaling pathways and regulates the expression of anti-microbial peptide genes, mainly relies on peptidoglycan recognition proteins (PGRPs) for the detection of bacterial pathogens. To explore the effect of zebrafish peptidoglycan recognition protein 6 (zfPGRP6) on Toll-like receptor signaling pathway, RNA interference (siRNA) and real time quantitative PCR (RQ-PCR) methods were used to identify differentially expressed genes regulated by zfPGRP6. The target genes included TLR2, TLR3, TLR5, TLR7, TLR8, IL1R, Sterile-alpha and Armadillo motif containing protein (SARM), myeloid differentiation factor 88 (MyD88) and nuclear factor (NF)-kappa B2 (p100/p52). The results of RQ-PCR showed that RNAi-mediated suppression of zfPGRP6 significantly down-regulated the expression of TLR2, TLR5, IL1R, SARM, MyD88 and p100/p52. The expression of beta-defensin-1 was also down-regulated in those embryos silenced by zfPGRP6. In challenge experiments to determine the anti-bacterial response to Gram-negative bacteria, RNAi knock-down of zfPGRP6 markedly increased susceptibility to Flavobacterium columnare.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping