PUBLICATION
Bio-electrosprayed multicellular zebrafish embryos are viable and develop normally
- Authors
- Clarke, J.D., and Jayasinghe, S.N.
- ID
- ZDB-PUB-080512-3
- Date
- 2008
- Source
- Biomedical materials (Bristol, England) 3(1): 11001 (Journal)
- Registered Authors
- Clarke, Jon
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Survival
- Electrochemistry/methods*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/physiology*
- Embryonic Development/physiology*
- Fetal Viability/physiology
- Microfluidics/methods*
- Organ Culture Techniques/methods*
- Pressure
- Tissue Engineering/methods
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- Zebrafish/physiology*
- PubMed
- 18458487 Full text @ Biomed. Mater.
Citation
Clarke, J.D., and Jayasinghe, S.N. (2008) Bio-electrosprayed multicellular zebrafish embryos are viable and develop normally. Biomedical materials (Bristol, England). 3(1):11001.
Abstract
Bio-electrosprays are rapidly emerging as a viable protocol for directly engineering living cells. This communication reports the bio-electrospraying of multicellular organisms, namely zebrafish embryos. The results demonstrate that the bio-electrospray protocol fails to induce any embryological perturbations. In addition to analysing overall embryo morphology, we use transgenic embryos that express green fluorescent protein in specific brain neurons to determine that neuronal numbers and organization are completely normal. These results demonstrate that the bio-electrospraying protocol does not interfere with the complex gene regulation and cell movements required for the development of a multicellular organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping