Exposure to 17α-ethynylestradiol impairs reproductive functions of both male and female zebrafish (Danio rerio)
- Xu, H., Yang, J., Wang, Y., Jiang, Q., Chen, H., and Song, H.
- Aquatic toxicology (Amsterdam, Netherlands) 88(1): 1-8 (Journal)
- Registered Authors
- Chen, Hao, Jiang, Qiu, Song, Houyan, Wang, Yuexiang, Xu, Hui
- Zebrafish, 17α-Ethynylestradiol, Gonad development, Reproduction
- MeSH Terms
- Ethinyl Estradiol/toxicity*
- Ovary/drug effects
- Reproduction/drug effects*
- Sex Ratio
- Spermatozoa/drug effects
- Survival Analysis
- Testis/drug effects
- Water Pollutants, Chemical/toxicity*
- Zebrafish/growth & development
- 18453010 Full text @ Aquat. Toxicol.
Xu, H., Yang, J., Wang, Y., Jiang, Q., Chen, H., and Song, H. (2008) Exposure to 17α-ethynylestradiol impairs reproductive functions of both male and female zebrafish (Danio rerio). Aquatic toxicology (Amsterdam, Netherlands). 88(1):1-8.
In this study, the impact of 17alpha-ethynylestradiol (EE(2)) on reproduction in zebrafish (Danio rerio) was evaluated using vitellogenin (Vtg) induction, mortality rate, growth, sex ratio, gonad histology, fecundity, and sperm parameters as endpoints. Two days post-hatch (2dph) zebrafish were exposed to solvent control or EE(2) at 0.4, 2, and 10ng/l for 3 months. At 21dph, Vtg mRNA expression was detected only in fish exposed to 10ng/l EE(2). At 90dph, increased mortality rate and sex ratio (female:male) were observed in fish exposed to 2 and 10ng/l EE(2). A dose-dependent increase in gonads with underdeveloped gametes was observed in fish exposed to EE(2). At 180dph, malformation of the sperm duct and reduced number of spermatozoa were found in fish exposed to 2ng/l and 10ng/l EE(2). Reduced fecundity and 12hpf egg viability were found in EE(2)-exposed males and females. The number of fish with no expressible milt was elevated dose dependently in EE(2)-exposed males, although no difference in sperm density was found. After a 3-month recovery period, growth and sex ratio were partially recovered. Our findings suggest that EE(2) can adversely affect the fecundity, sex differentiation, gametes development, and other reproductive functions of both male and female zebrafish, and some of the toxic effects persist.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes