ZFIN ID: ZDB-PUB-080506-13
The endosomal protein Appl1 mediates Akt substrate specificity and cell survival in vertebrate development
Schenck, A., Goto-Silva, L., Collinet, C., Rhinn, M., Giner, A., Habermann, B., Brand, M., and Zerial, M.
Date: 2008
Source: Cell   133(3): 486-497 (Journal)
Registered Authors: Brand, Michael, Rhinn, Muriel
MeSH Terms:
  • Animals
  • Apoptosis
  • Cell Survival*
  • Embryonic Development
  • Endosomes/chemistry
  • Gene Expression Regulation, Developmental
  • Glycogen Synthase Kinase 3/metabolism
  • Molecular Sequence Data
  • Organ Specificity
  • Proto-Oncogene Proteins c-akt/metabolism*
  • Signal Transduction
  • Substrate Specificity
  • Vertebrates
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/analysis
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 18455989 Full text @ Cell
During development of multicellular organisms, cells respond to extracellular cues through nonlinear signal transduction cascades whose principal components have been identified. Nevertheless, the molecular mechanisms underlying specificity of cellular responses remain poorly understood. Spatial distribution of signaling proteins may contribute to signaling specificity. Here, we tested this hypothesis by investigating the role of the Rab5 effector Appl1, an endosomal protein that interacts with transmembrane receptors and Akt. We show that in zebrafish, Appl1 regulates Akt activity and substrate specificity, controlling GSK-3beta but not TSC2. Consistent with this pattern, Appl1 is selectively required for cell survival, most critically in highly expressing tissues. Remarkably, Appl1 function requires its endosomal localization. Indeed, Akt and GSK-3beta, but not TSC2, dynamically associate with Appl1 endosomes upon growth factor stimulation. We propose that partitioning of Akt and selected effectors onto endosomal compartments represents a key mechanism contributing to the specificity of signal transduction in vertebrate development.