PUBLICATION
The gonadotropin receptors FSH-R and LH-R of Atlantic halibut (Hippoglossus hippoglossus), 1: Isolation of multiple transcripts encoding full-length and truncated variants of FSH-R
- Authors
- Kobayashi, T., and Andersen, O.
- ID
- ZDB-PUB-080327-15
- Date
- 2008
- Source
- General and comparative endocrinology 156(3): 584-594 (Journal)
- Registered Authors
- Keywords
- Gonadotropin receptor, Atlantic halibut, Alternative splicing, Truncated receptor, Asynchronous oogenesis, Repetitive spawning
- MeSH Terms
-
- Animals
- Molecular Sequence Data
- Receptors, FSH/biosynthesis
- Receptors, FSH/genetics
- Receptors, FSH/metabolism*
- Receptors, LH/biosynthesis
- Receptors, LH/genetics
- Receptors, LH/metabolism*
- Alternative Splicing
- Female
- RNA, Messenger/biosynthesis
- RNA, Messenger/genetics
- Base Sequence
- Flounder/physiology*
- Cloning, Molecular
- Amino Acid Sequence
- Male
- PubMed
- 18359484 Full text @ Gen. Comp. Endocrinol.
Citation
Kobayashi, T., and Andersen, O. (2008) The gonadotropin receptors FSH-R and LH-R of Atlantic halibut (Hippoglossus hippoglossus), 1: Isolation of multiple transcripts encoding full-length and truncated variants of FSH-R. General and comparative endocrinology. 156(3):584-594.
Abstract
As a first step towards understanding the regulatory mechanisms underlying the asynchronous oogenesis in repetitive spawning fish, full-length cDNAs encoding the receptors for follicle stimulating hormone (FSH-R) and luteinizing hormone (LH-R) were isolated from the gonads of the flatfish Atlantic halibut (Hippoglossus hippoglossus). The predicted halibut FSH-R and LH-R of 664 and 698 amino acids, respectively, both contain the characteristic features of a large extracellular (EC) domain, a hepta-helical transmembrane (TM) domain, and a short cytoplasmic C-terminal tail. Halibut FSH-R and LH-R share only 42% overall sequence identity mostly due to low homology in the ligand-binding EC domain. Both receptors lular (EC) domain, a hepta-helical transmembrane (TM) domain, and a short cytoplasmic C-terminal tail. Halibut FSH-R and LH-R share only show high sequence identity to their orthologs of Nile tilapia, but seem to be more remotely related to the receptors in catfish, zebrafish and salmonids. In contrast to the intron-less TM domain of almost all vertebrate gonadotropin receptors, three introns were identified in this domain of halibut FSH-R, thus resembling the gene structure of Drosophila glycoprotein hormone receptor type I. The FSH-R pre-mRNA was shown to be processed in alternative ways by isolating two different transcripts encoding the complete receptor and four alternative spliced transcripts encoding different truncated receptor variants. Based on the DNA sequence variation and chromosomal organization of the gonadotropin receptors in several teleosts, we propose that the encoding genes have been duplicated in the fish lineage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping