PUBLICATION
Shuttling of the chaperones Unc45b and Hsp90a between the A band and the Z line of the myofibril
- Authors
- Etard, C., Roostalu, U., and Strähle, U.
- ID
- ZDB-PUB-080326-26
- Date
- 2008
- Source
- The Journal of cell biology 180(6): 1163-1175 (Journal)
- Registered Authors
- Etard, Christelle, Roostalu, Urmas, Strähle, Uwe
- Keywords
- none
- MeSH Terms
-
- Animals
- HSP90 Heat-Shock Proteins/metabolism*
- Intracellular Membranes/metabolism*
- Intracellular Membranes/ultrastructure
- Models, Biological
- Molecular Chaperones/metabolism*
- Muscle Fibers, Skeletal/metabolism*
- Muscle Fibers, Skeletal/ultrastructure
- Muscle, Striated/injuries
- Muscle, Striated/metabolism*
- Muscle, Striated/ultrastructure
- Myosins/metabolism
- Protein Binding/physiology
- Protein Structure, Tertiary/physiology
- Protein Transport/physiology
- Regeneration/physiology
- Stress, Mechanical
- Time Factors
- Zebrafish
- Zebrafish Proteins/metabolism*
- PubMed
- 18347070 Full text @ J. Cell Biol.
Citation
Etard, C., Roostalu, U., and Strähle, U. (2008) Shuttling of the chaperones Unc45b and Hsp90a between the A band and the Z line of the myofibril. The Journal of cell biology. 180(6):1163-1175.
Abstract
The formation of thick filaments in striated muscle involves the chaperones Hsp90a and Unc45. We show that Unc45b and Hsp90a, two zebrafish orthologues, colocalize with myosin during myofibrillogenesis and associate with the Z line when myofibril assembly is completed. In response to stress or damage to the myofiber, Unc45b and Hsp90a dissociate from the Z line and transiently associate with myosin. Although chaperone activity of Unc45b requires the full-length protein, only the central and Unc45-Cro1p-She4p domains are required to anchor it to the Z line, and multiple subdomains mediate association with nascent myosin. We propose that the Z line serves as a reservoir for chaperones, allowing a rapid mobilization in response to muscle damage. Our data are consistent with a differential affinity model as an explanation for the shuttling of the chaperones between the Z line and myosin.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping