PUBLICATION
Co-injection strategies to modify radiation sensitivity and tumor initiation in transgenic Zebrafish
- Authors
- Langenau, D.M., Keefe, M.D., Storer, N.Y., Jette, C.A., Smith, A.C., Ceol, C.J., Bourque, C., Look, A.T., and Zon, L.I.
- ID
- ZDB-PUB-080326-25
- Date
- 2008
- Source
- Oncogene 27(30): 4242-4248 (Journal)
- Registered Authors
- Bourque, Caitlin, Ceol, Craig, Jette, Cicely A., Keefe, Matthew, Langenau, David, Look, A. Thomas, Smith, Amanda, Storer, Narie, Zon, Leonard I.
- Keywords
- Myc, Ras, heat-shock, T-cell acute lymphoblastic leukemia, rhabdomyosarcoma
- MeSH Terms
-
- Cleavage Stage, Ovum
- Proto-Oncogene Proteins/genetics
- Animals, Genetically Modified
- Cell Transformation, Neoplastic/genetics*
- Cell Transformation, Neoplastic/radiation effects*
- Genes, bcl-2
- DNA-Binding Proteins/administration & dosage
- DNA-Binding Proteins/genetics
- Mutant Proteins/genetics
- Embryo, Nonmammalian
- Animals
- Microinjections/methods*
- Transgenes
- Zebrafish/embryology
- ras Proteins/genetics
- Green Fluorescent Proteins/administration & dosage
- Green Fluorescent Proteins/genetics
- Genes, myc
- Neoplasms, Radiation-Induced/genetics*
- Nuclear Proteins/administration & dosage
- Nuclear Proteins/genetics
- Gene Transfer Techniques*
- Luminescent Proteins/administration & dosage
- Luminescent Proteins/genetics
- Genes, p53
- Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/genetics
- PubMed
- 18345029 Full text @ Oncogene
Citation
Langenau, D.M., Keefe, M.D., Storer, N.Y., Jette, C.A., Smith, A.C., Ceol, C.J., Bourque, C., Look, A.T., and Zon, L.I. (2008) Co-injection strategies to modify radiation sensitivity and tumor initiation in transgenic Zebrafish. Oncogene. 27(30):4242-4248.
Abstract
The zebrafish has emerged as a powerful genetic model of cancer, but has been limited by the use of stable transgenic approaches to induce disease. Here, a co-injection strategy is described that capitalizes on both the numbers of embryos that can be microinjected and the ability of transgenes to segregate together and exert synergistic effects in forming tumors. Using this mosaic transgenic approach, gene pathways involved in tumor initiation and radiation sensitivity have been identified.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping