PUBLICATION

Co-injection strategies to modify radiation sensitivity and tumor initiation in transgenic Zebrafish

Authors
Langenau, D.M., Keefe, M.D., Storer, N.Y., Jette, C.A., Smith, A.C., Ceol, C.J., Bourque, C., Look, A.T., and Zon, L.I.
ID
ZDB-PUB-080326-25
Date
2008
Source
Oncogene   27(30): 4242-4248 (Journal)
Registered Authors
Bourque, Caitlin, Ceol, Craig, Jette, Cicely A., Keefe, Matthew, Langenau, David, Look, A. Thomas, Smith, Amanda, Storer, Narie, Zon, Leonard I.
Keywords
Myc, Ras, heat-shock, T-cell acute lymphoblastic leukemia, rhabdomyosarcoma
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Transformation, Neoplastic/genetics*
  • Cell Transformation, Neoplastic/radiation effects*
  • Cleavage Stage, Ovum
  • DNA-Binding Proteins/administration & dosage
  • DNA-Binding Proteins/genetics
  • Embryo, Nonmammalian
  • Gene Transfer Techniques*
  • Genes, bcl-2
  • Genes, myc
  • Genes, p53
  • Green Fluorescent Proteins/administration & dosage
  • Green Fluorescent Proteins/genetics
  • Luminescent Proteins/administration & dosage
  • Luminescent Proteins/genetics
  • Microinjections/methods*
  • Mutant Proteins/genetics
  • Neoplasms, Radiation-Induced/genetics*
  • Nuclear Proteins/administration & dosage
  • Nuclear Proteins/genetics
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/genetics
  • Proto-Oncogene Proteins/genetics
  • Transgenes
  • Zebrafish/embryology
  • ras Proteins/genetics
PubMed
18345029 Full text @ Oncogene
Abstract
The zebrafish has emerged as a powerful genetic model of cancer, but has been limited by the use of stable transgenic approaches to induce disease. Here, a co-injection strategy is described that capitalizes on both the numbers of embryos that can be microinjected and the ability of transgenes to segregate together and exert synergistic effects in forming tumors. Using this mosaic transgenic approach, gene pathways involved in tumor initiation and radiation sensitivity have been identified.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes