PUBLICATION

Anandamide modulates growth and lipid metabolism in the zebrafish Danio rerio

Authors
Migliarini, B., and Carnevali, O.
ID
ZDB-PUB-080326-19
Date
2008
Source
Molecular and Cellular Endocrinology   286(1-2):S1: S12-S16 (Journal)
Registered Authors
Carnevali, Oliana
Keywords
Endocannabinoids, AM251, CB1, IGFs, SREBP
MeSH Terms
  • Animals
  • Arachidonic Acids/pharmacology*
  • Endocannabinoids
  • Gene Expression Regulation, Developmental/drug effects
  • Insulin-Like Growth Factor I/genetics
  • Insulin-Like Growth Factor I/metabolism
  • Insulin-Like Growth Factor II/genetics
  • Insulin-Like Growth Factor II/metabolism
  • Larva/drug effects
  • Larva/metabolism
  • Lipid Metabolism/drug effects*
  • Liver/drug effects
  • Liver/metabolism
  • Piperidines/pharmacology
  • Polyunsaturated Alkamides/pharmacology*
  • Pyrazoles/pharmacology
  • Receptor, Cannabinoid, CB1/genetics
  • Receptor, Cannabinoid, CB1/metabolism
  • Sterol Regulatory Element Binding Proteins/genetics
  • Sterol Regulatory Element Binding Proteins/metabolism
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish/metabolism*
PubMed
18342434 Full text @ Mol. Cell. Endocrinol.
Abstract
Endocannabinoids are known to be lipidic mediators with several biological functions as the stimulation of food intake and lipid metabolism via cannabinoid receptor CB1. Many evidences, such as the presence of CB1 mRNA in fat tissue, suggest a peripheral role for endocannabinoids in regulating lipogenesis and body weight in mammals. As animal models constitute good tools to study endocannabinoid system dynamics, we analyzed the role of the endocannabinoid anandamide (AEA) in modulating lipid metabolism and growth in zebrafish larvae and adults. The data obtained indicated that AEA administered via water modulates the transcription of its own receptor CB1, besides to up-regulate gene expression of sterol regulatory element binding protein (SREBP) and of the insulin-like growth factors (IGF-1 and IGF-2). The results here obtained represent the first evidence in fish of the endocannabinoid system involvement in lipid metabolism and growth.
Genes / Markers
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Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
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Mapping