ZFIN ID: ZDB-PUB-080309-20
Cloning and expression of translation elongation factor 2 (EF-2) in zebrafish
Zhang, S.H., Yao, J.H., Song, H.D., Wang, L., and Xue, J.L.
Date: 2008
Source: DNA sequence : the journal of DNA sequencing and mapping   19(1): 1-7 (Journal)
Registered Authors: Wang, Lu
Keywords: EF-2, translation elongation factor 2, zebrafish, whole-mount in situ hybridization, expression
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Cricetinae
  • Gene Expression Regulation, Developmental*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Peptide Elongation Factor 2/biosynthesis*
  • Peptide Elongation Factor 2/genetics*
  • Transcription, Genetic
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics*
PubMed: 18300156 Full text @ DNA Seq.
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ABSTRACT
We have identified a developmentally regulated gene translation elongation factor 2 (EF-2) in zebrafish (GenBank Accession No. AAQ91234). Analysis of DNA sequence of zebrafish EF-2 shows that the 2826 bp cDNA spans an open reading frame from nucleotide 55 to 2631 and encodes a protein of 858 amino acids. It shares an identity of 92, 93, 93, 92, 79 and 80% in amino acid sequence to human, mouse, Chinese hamster, Gallus gullus, C. elegans and Drosophila EF-2, respectively. Zebrafish EF-2 protein has 16 conserved domains, GTP-binding domain is found in the NH(2) terminus, and the ADP-ribosylation domain locates at the COOH terminus. Whole mount in situ hybridization on zebrafish embryos shows that the transcripts of EF-2 gene are detected during the early development of zebrafish embryo and constantly change from 5-somite stage to protruding-mouth stage. It expresses strongly throughout envelope at 5-somite stage. Then the stained cells concentrate strongly in the eyes, brain and muscle tissue. From prim-25 stage the stained cells only appear strongly in the lens and the anterior portion of the cerebellum.
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