PUBLICATION
Systolic and diastolic ventricular function in zebrafish embryos: influence of norepenephrine, MS-222 and temperature
- Authors
- Denvir, M.A., Tucker, C.S., and Mullins, J.J.
- ID
- ZDB-PUB-080306-28
- Date
- 2008
- Source
- BMC Biotechnology 8: 21 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Aminobenzoates/pharmacology
- Video Recording/methods*
- Systole/drug effects*
- Systole/physiology*
- Temperature
- Norepinephrine/pharmacology
- Ventricular Function/drug effects*
- Ventricular Function/physiology*
- Embryonic Development/drug effects*
- Embryonic Development/physiology*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/physiology
- Adrenergic alpha-Agonists/pharmacology
- In Vitro Techniques
- Zebrafish/embryology*
- Zebrafish/physiology
- Anesthetics/pharmacology
- Movement/drug effects
- Movement/physiology
- Diastole/drug effects*
- Diastole/physiology*
- Heart Ventricles/drug effects
- Heart Ventricles/embryology
- Microscopy, Interference/methods
- Image Enhancement/instrumentation
- Image Enhancement/methods
- PubMed
- 18304347 Full text @ BMC Biotechnol.
Citation
Denvir, M.A., Tucker, C.S., and Mullins, J.J. (2008) Systolic and diastolic ventricular function in zebrafish embryos: influence of norepenephrine, MS-222 and temperature. BMC Biotechnology. 8:21.
Abstract
BACKGROUND: Zebrafish are increasingly used to study the influences of gene mutation and manipulation on cardiac development, structure and function. In this study, a video edge detection system was used to characterise, continuously, cardiac ventricle function in 2-5 days old zebrafish embryos embedded in 0.6% agar and examined under light microscopy at room temperature (22 degrees centigrade). Using video edge detection software (IonOptix Inc), the motion of a small region of the cardiac ventricle wall was converted to a continuous chart trace allowing analysis of wall motion amplitude (WMA) and myocardial wall velocity during systole (MWVs) and diastole (MWVd). RESULTS: Cardiac wall motion characteristics changed progressively from day 2 to 5 (WMA, 2-days, 17.6+/-4.4 vs 5-days, 24.6+/-4.7 micrometers , p<0.01). MWVd was more rapid than MWVs at all developmental time points. Embryonic hearts were also assessed after increasing concentrations of norepenephrine (NE) and the anaesthetic agent MS222 (tricaine) were added to the bathing water. In response to NE, WMA increased significantly more in 4 day embryos compared with 2 day embryos (change in WMA,13.6+/-8.2 vs 4.0+/-8.8 micrometers, p=0.01, respectively) while the decrease in WMA in response to MS222 was similar in both 2 and 4-day embryos. Heart rate, MWVs and MWVd were significantly higher at 28 compared with 22 degrees centigrade. No differences in cardiac function were observed between AB and Golden strains. CONCLUSIONS: Video edge detection appears sufficiently sensitive to detect subtle changes in diastolic and systolic cardiac function during development and changes resulting from pharmacological and environmental interventions. Such measurements could be valuable in assessment of altered cardiac function after genetic manipulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping