PUBLICATION

Identification of ZBP-89 as a Novel GATA-1 Associated Transcription Factor Involved in Megakaryocytic and Erythroid Development

Authors
Woo, A.J., Moran, T.B., Schindler, Y., Choe, S.K., Langer, N.B., Sullivan, M.R., Fujiwara, Y., Paw, B.H., and Cantor, A.B.
ID
ZDB-PUB-080227-7
Date
2008
Source
Molecular and cellular biology   28(8): 2675-2689 (Journal)
Registered Authors
Choe, Seong-Kyu, Paw, Barry
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation*
  • Cell Line
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/isolation & purification
  • DNA-Binding Proteins/metabolism*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Erythroid Cells/cytology
  • Erythroid Cells/metabolism*
  • GATA1 Transcription Factor/chemistry
  • GATA1 Transcription Factor/genetics
  • GATA1 Transcription Factor/isolation & purification
  • GATA1 Transcription Factor/metabolism*
  • Megakaryocytes/cytology
  • Megakaryocytes/metabolism*
  • Mice
  • Molecular Sequence Data
  • Ploidies
  • Protein Binding
  • Rats
  • Transcription Factors/genetics
  • Transcription Factors/isolation & purification
  • Transcription Factors/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
18250154 Full text @ Mol. Cell. Biol.
Abstract
A complete understanding of the transcriptional regulation of developmental lineages requires that all relevant factors be identified. Here, we have taken a proteomic approach to identify novel proteins associated with GATA-1, a lineage-restricted zinc finger transcription factor required for terminal erythroid and megakaryocytic maturation. We identify the Krüppel-type zinc finger transcription factor ZBP-89 as a component of multiprotein complexes involving GATA-1 and its essential cofactor Friend of GATA-1 (FOG-1). Using chromatin immunoprecipitation assays, we show that GATA-1 and ZBP-89 co-occupy cis-regulatory elements of certain erythroid and megakaryocyte-specific genes, including an enhancer of the GATA-1 gene itself. Loss-of-function studies in zebrafish and mice demonstrate an in vivo requirement for ZBP-89 in megakaryopoiesis and definitive erythropoiesis, but not primitive erythropoiesis, phenocopying aspects of FOG-1 and GATA-1 deficient animals. These findings identify ZBP-89 as a novel transcription factor involved in erythroid and megakaryocytic development and suggest that it serves a cooperative function with GATA-1 and/or FOG-1 in a developmental stage-specific manner.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping