PUBLICATION

A mutation in the cone-specific pde6 gene causes rapid cone photoreceptor degeneration in zebrafish

Authors
Stearns, G., Evangelista, M., Fadool, J.M., and Brockerhoff, S.E.
ID
ZDB-PUB-071219-20
Date
2007
Source
The Journal of neuroscience : the official journal of the Society for Neuroscience   27(50): 13866-13874 (Journal)
Registered Authors
Brockerhoff, Susan, Fadool, James M.
Keywords
zebrafish, photoreceptor, phototransduction, retina, phosphodiesterase, degeneration
MeSH Terms
  • Animals
  • Cell Count
  • Cyclic Nucleotide Phosphodiesterases, Type 6/biosynthesis
  • Cyclic Nucleotide Phosphodiesterases, Type 6/genetics*
  • Disease Models, Animal*
  • Disease Progression
  • Electroretinography
  • Genes, Recessive
  • Mutation/genetics*
  • Retinal Cone Photoreceptor Cells/enzymology*
  • Retinal Cone Photoreceptor Cells/pathology
  • Retinal Degeneration/genetics*
  • Retinal Degeneration/pathology
  • Retinal Rod Photoreceptor Cells/pathology
  • Zebrafish
PubMed
18077698 Full text @ J. Neurosci.
Abstract
Photoreceptor degeneration is a common cause of inherited blindness worldwide. We have identified a blind zebrafish mutant with rapid degeneration of cone photoreceptors caused by a mutation in the cone phosphodiesterase c (pde6c) gene, a key regulatory component in cone phototransduction. Some rods also degenerate, primarily in areas with a low density of rods. Rod photoreceptors in areas of the retina that always have a high density of rods are protected from degeneration. Our findings demonstrate that, analogous to what happens to rod photoreceptors in the rd1 mouse model, loss of cone phosphodiesterase leads to rapid degeneration of cone photoreceptors. Furthermore, we propose that cell density plays a key role in determining whether rod photoreceptors degenerate as a secondary consequence to cone degeneration. Our zebrafish mutant serves as a model for developing therapeutic treatments for photoreceptor degeneration in humans.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping