PUBLICATION

Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and disrupts zebrafish digestive development

Authors
Yee, N.S., Gong, W., Huang, Y., Lorent, K., Dolan, A.C., Maraia, R.J., and Pack, M.
ID
ZDB-PUB-071210-2
Date
2007
Source
PLoS Biology   5(11): e312 (Journal)
Registered Authors
Yee, Nelson S.
Keywords
none
MeSH Terms
  • Animals
  • Cell Proliferation/drug effects
  • Digestive System/drug effects
  • Digestive System/enzymology
  • Digestive System/growth & development*
  • Gene Expression Regulation, Developmental*
  • Larva/drug effects
  • Larva/enzymology*
  • Larva/growth & development
  • Mutation
  • Oligonucleotides, Antisense/pharmacology
  • RNA Polymerase III/genetics*
  • RNA Polymerase III/metabolism
  • Zebrafish/physiology*
PubMed
18044988 Full text @ PLoS Biol.
Abstract
The role of RNA polymerase III (Pol III) in developing vertebrates has not been examined. Here, we identify a causative mutation of the second largest Pol III subunit, polr3b, that disrupts digestive organ development in zebrafish slim jim (slj) mutants. The slj mutation is a splice-site substitution that causes deletion of a conserved tract of 41 amino acids in the Polr3b protein. Structural considerations predict that the slj Pol3rb deletion might impair its interaction with Polr3k, the ortholog of an essential yeast Pol III subunit, Rpc11, which promotes RNA cleavage and Pol III recycling. We engineered Schizosaccharomyces pombe to carry an Rpc2 deletion comparable to the slj mutation and found that the Pol III recovered from this rpc2-delta yeast had markedly reduced levels of Rpc11p. Remarkably, overexpression of cDNA encoding the zebrafish rpc11 ortholog, polr3k, rescued the exocrine defects in slj mutants, indicating that the slj phenotype is due to deficiency of Rpc11. These data show that functional interactions between Pol III subunits have been conserved during eukaryotic evolution and support the utility of zebrafish as a model vertebrate for analysis of Pol III function.
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Human Disease / Model
Sequence Targeting Reagents
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Engineered Foreign Genes
Mapping