|ZFIN ID: ZDB-PUB-071129-1|
Differential expression of duplicated VAL-opsin genes in the developing zebrafish
Kojima, D., Torii, M., Fukada, Y., and Dowling, J.E.
|Source:||Journal of neurochemistry 104(5): 1364-1371 (Journal)|
|Registered Authors:||Dowling, John E., Fukada, Yoshitaka, Kojima, Daisuke|
|PubMed:||18036148 Full text @ J. Neurochem.|
Kojima, D., Torii, M., Fukada, Y., and Dowling, J.E. (2008) Differential expression of duplicated VAL-opsin genes in the developing zebrafish. Journal of neurochemistry. 104(5):1364-1371.
ABSTRACTNon-visual opsins mediate various light-dependent physiological events. Our previous search for non-visual opsin genes in zebrafish led to the discovery of VAL-opsin (VAL-opsinA) in deep brain cells and retinal horizontal cells of the adult fish. In this study, we report the identification and characterization of its duplicated gene, VAL-opsinB, in zebrafish. A molecular phylogenetic analysis indicates that VAL-opsinB is orthologous to a previously reported salmon gene and that the duplication of the VAL-opsin gene occurred in the teleost lineage. The recombinant protein of zebrafish VAL-opsinB forms a green-sensitive photopigment when reconstituted with 11-cis-retinal. VAL-opsinB expression was detected in a limited number of cells of the brain and the eye, and the expression pattern is distinct from that of the VAL-opsinA gene. Such a differential expression pattern suggests that VAL-opsinA and VAL-opsinB are involved in different physiological events in zebrafish.