|ZFIN ID: ZDB-PUB-071125-3|
Otoc1: A novel otoconin-90 ortholog required for otolith mineralization in zebrafish
Petko, J.A., Millimaki, B.B., Canfield, V.A., Riley, B.B., and Levenson, R.
|Source:||Developmental Neurobiology 68(2): 209-222 (Journal)|
|Registered Authors:||Canfield, Victor, Levenson, Robert, Riley, Bruce|
|Keywords:||Reissner's Substance, zebrafish, otoconin, Otoc1, otolith, matrix protein|
|PubMed:||18000829 Full text @ Dev. Neurobiol.|
Petko, J.A., Millimaki, B.B., Canfield, V.A., Riley, B.B., and Levenson, R. (2008) Otoc1: A novel otoconin-90 ortholog required for otolith mineralization in zebrafish. Developmental Neurobiology. 68(2):209-222.
ABSTRACTWithin the vestibular system of virtually all vertebrate species, gravity and linear acceleration are detected via coupling of calcified masses to the cilia of mechanosensory hair cells. The mammalian ear contains thousands of minute biomineralized particles called otoconia, whereas the inner ear of teleost fish contains three large ear stones called otoliths that serve a similar function. Otoconia and otoliths are composed of calcium carbonate crystals condensed on a core protein lattice. Otoconin-90 (Oc90) is the major matrix protein of mammalian and avian otoconia, while otolith matrix protein (OMP) is the most abundant matrix protein found in the otoliths of teleost fish. We have identified a novel gene, otoc1, which encodes the zebrafish ortholog of Oc90. Expression of otoc1 was detected in the ear between 15 hpf and 72 hpf, and was restricted primarily to the macula and the developing epithelial pillars of the semicircular canals. Expression of otoc1 was also detected in epiphysis, optic stalk, midbrain, diencephalon, flexural organ, and spinal cord. During embryogenesis, expression of otoc1 mRNA preceded the appearance of omp-1 transcripts. Knockdown of otoc1 mRNA translation with antisense morpholinos produced a variety of aberrant otolith phenotypes. Our results suggest that Otoc1 may serve to nucleate calcium carbonate mineralization of aragonitic otoliths.