Unexpected Novel Relational Links Uncovered by Extensive Developmental Profiling of Nuclear Receptor Expression

Bertrand, S., Thisse, B., Tavares, R., Sachs, L., Chaumot, A., Bardet, P.L., Escrivà, H., Duffraisse, M., Marchand, O., Safi, R., Thisse, C., and Laudet, V.
PLoS Genetics   3(11): e188 (Journal)
Registered Authors
Bardet, Pierre-Luc, Bertrand, Stéphanie, Laudet, Vincent, Marchand, Oriane, Safi, Rachid, Thisse, Bernard, Thisse, Christine
Gene expression, Retina, Zebrafish, Evolutionary genetics, Embryos, Central nervous system, Sequence alignment, In situ hybridization
MeSH Terms
  • Animals
  • Brain/embryology
  • Brain/metabolism
  • DNA, Complementary
  • Embryo, Nonmammalian/embryology
  • Gene Duplication
  • Gene Expression Profiling*
  • Gene Expression Regulation, Developmental*
  • Humans
  • In Situ Hybridization
  • Phylogeny
  • Receptors, Cytoplasmic and Nuclear/genetics*
  • Retina/embryology
  • Retina/metabolism
  • Retinoid X Receptors/genetics
  • Sequence Analysis, DNA
  • Sequence Analysis, Protein
  • Zebrafish/embryology*
  • Zebrafish/genetics*
17997606 Full text @ PLoS Genet.
Nuclear receptors (NRs) are transcription factors that are implicated in several biological processes such as embryonic development, homeostasis, and metabolic diseases. To study the role of NRs in development, it is critically important to know when and where individual genes are expressed. Although systematic expression studies using reverse transcriptase PCR and/or DNA microarrays have been performed in classical model systems such as Drosophila and mouse, no systematic atlas describing NR involvement during embryonic development on a global scale has been assembled. Adopting a systems biology approach, we conducted a systematic analysis of the dynamic spatiotemporal expression of all NR genes as well as their main transcriptional coregulators durint during embryonic development on a global scale has been assembled. Adopting a systems biology approach, we conducted a systematic analysis of theg zebrafish development (101 genes) using whole-mount in situ hybridization. This extensive dataset establishes overlapping expression patterns among NRs and coregulators, indicating hierarchical transcriptional networks. This complete developmental profiling provides an unprecedented examination of expression of NRs during embryogenesis, uncovering their potential function during central nervous system and retina formation. Moreover, our study reveals that tissue specificity of hormone action is conferred more by the receptors than by their coregulators. Finally, further evolutionary analyses of this global resource led us to propose that neofunctionalization of duplicated genes occurs at the levels of both protein sequence and RNA expression patterns. Altogether, this expression database of NRs provides novel routes for leading investigation into the biological function of each individual NR as well as for the study of their combinatorial regulatory circuitry within the superfamily.
Errata / Notes
Data from Figure S3 and Table S3 directly submitted by Thisse B. and Thisse C. 2008.
Genes / Markers
Show all Figures
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes