PUBLICATION

Abundant expression of nucleosome assembly protein 1 (nap1) gene in goldfish scale with lateral line

Authors
Li, L., Katsuyama, H., DO, S.N., Saito, M., Tanii, H., and Saijoh, K.
ID
ZDB-PUB-071118-12
Date
2007
Source
The Journal of Toxicological Sciences   32(4): 359-365 (Journal)
Registered Authors
Katsuyama, Hironobu
Keywords
none
MeSH Terms
  • RNA/genetics
  • Environmental Monitoring/methods*
  • Molecular Sequence Data
  • Cell Cycle Proteins/genetics*
  • Animals
  • In Situ Hybridization
  • DNA, Complementary/genetics
  • Nuclear Proteins/genetics*
  • Goldfish/anatomy & histology
  • Goldfish/genetics*
  • Amino Acid Sequence
  • Gene Expression/drug effects*
  • Nucleosome Assembly Protein 1
  • Integumentary System*
  • Lateral Line System/drug effects*
  • Lateral Line System/metabolism
  • Base Sequence
  • Environmental Pollutants/toxicity*
  • Gills/drug effects
  • Gills/metabolism
(all 20)
PubMed
17965550 Full text @ J. Toxicol. Sci.
Abstract
To explore the possible utilization of goldfish scale in monitoring environmental toxicants as well as that for calcified tissue research, characteristics of cells in the scale were examined by identifying genes differentially expressed in those cells. Subtraction cloning was subjected to RNAs between scale - gill (SG), gill - scale (GS) and scale with lateral line - scale without lateral line (LS). Total numbers of 4, 6, and 9 clones were isolated respectively from SG, GS and LS pools. Blast search of their sequence showed only low homology to other fish sequences so that 5' rapid amplification of cDNA (5'RACE) was applied to the obtained 5' terminal side of each sequence. Among 19 clones, LS3 alone showed the high homology to zebrafish nucleosome assembly protein 1 (NAP1) and the rest of 18 was not yet identified. In situ hybridization confirmed that faint expression of NAP1 mRNA was observed in cells along the ridge of the scale without lateral line. On the other hand, in small cells not along the ridge of the scale with lateral line, very intense hybridization was found as expected. They were abundant at the surrounding area of the lateral line tube. NAP1 plays an enhancing role on gene expression by promoting nucleosome assembly. Thus, cell viability in the scale with lateral line seemed to be higher than that in the scale without lateral line. The scale can be a good model to monitor the effects of environmental pollutants because simultaneous observation against cells with different cell viability is available through comparison between cells in the scale with or without lateral line.
Genes / Markers
Figures
No images available
Expression
Phenotype
No data available
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Li, L., Katsuyama, H., DO, S.N., Saito, M., Tanii, H., and Saijoh, K. (2007) Abundant expression of nucleosome assembly protein 1 (nap1) gene in goldfish scale with lateral line. The Journal of Toxicological Sciences. 32(4):359-365.