PUBLICATION
Characterization of the enigma family in zebrafish
- Authors
- Ott, E.B., Sakalis, P.A., Marques, I.J., and Bagowski, C.P.
- ID
- ZDB-PUB-071023-5
- Date
- 2007
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 236(11): 3144-3154 (Journal)
- Registered Authors
- Bagowski, Christoph P., Ott, Elisabeth B.
- Keywords
- LIM domain, PDZ, PDLIM, LIM mineralization protein, Enigma, Cypher, ZASP, LDB3, LMP1, Enigma Homolog, ENH, gene expression, zebrafish, development, embryogenesis
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing/genetics*
- Animals
- Cytoskeletal Proteins
- Cytoskeleton/metabolism
- Embryo, Nonmammalian/metabolism*
- Embryonic Development/genetics
- Gene Expression Profiling
- Gene Expression Regulation, Developmental*
- Homeodomain Proteins/genetics*
- Humans
- In Situ Hybridization
- Intracellular Signaling Peptides and Proteins/genetics
- LIM Domain Proteins
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- Protein Structure, Tertiary
- Somites/embryology
- Somites/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 17937393 Full text @ Dev. Dyn.
Citation
Ott, E.B., Sakalis, P.A., Marques, I.J., and Bagowski, C.P. (2007) Characterization of the enigma family in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 236(11):3144-3154.
Abstract
The three Enigma subfamily proteins, Enigma, Enigma homologue, and Cypher/ZASP belong to the PDZ and LIM encoding protein family, which is characterized by the presence of a PDZ- and one or more LIM domains. PDZ/LIM proteins play important biological roles, and all members have been shown to associate with the actin cytoskeleton. We describe here the splice form specific expression patterns for the three Enigma subfamily members during zebrafish embryogenesis. Whole-mount in situ hybridization revealed common and distinct expression patterns for the different PDZ or LIM domain encoding splice variants. We further studied the role of enigma in zebrafish development. Enigma knockdown appeared to be embryonic lethal shortly after the end of gastrulation and in few surviving embryos led to elongation defects and disorganized somites. In summary, we show here the temporal and spatial expression patterns of the three Enigma family members and their PDZ and LIM domain encoding splice forms during zebrafish embryogenesis. Our results suggest that enigma is important for the formation and organization of somites and might play an important role for actin cytoskeleton organization during development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping