|ZFIN ID: ZDB-PUB-071016-13|
Metabolic rate and reactive oxygen species production in different genotypes of GH-transgenic zebrafish
da Rosa, C.E., Figueiredo, M.A., Lanes, C.F., Almeida, D.V., Monserrat, J.M., and Marins, L.F.
|Source:||Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 149(1): 209-214 (Journal)|
|Registered Authors:||Almeida, Daniela Volcan, da Rosa, Carlos Eduardo, Figueiredo, Marcio de Azevedo, Lanes, Carlos Frederico Ceccon, Marins, Luis Fernando|
|Keywords:||Growth hormone, Transgenic zebrafish, Oxygen consumption, Reactive oxygen species, Glutamate-cysteine ligase catalytic subunit|
|PubMed:||17931920 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.|
da Rosa, C.E., Figueiredo, M.A., Lanes, C.F., Almeida, D.V., Monserrat, J.M., and Marins, L.F. (2008) Metabolic rate and reactive oxygen species production in different genotypes of GH-transgenic zebrafish. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 149(1):209-214.
ABSTRACTGrowth hormone overexpression increases growth and consequently increases the metabolic rate in fishes. Therefore, the objective of this study was to evaluate the effects of growth hormone overexpression in zebrafish Danio rerio in terms of growth, oxygen consumption, reactive oxygen species production, lipid hydroperoxide content, antioxidant enzyme activity and glutamate-cysteine ligase catalytic subunit gene expression. The employed models were wild type and transgenic (hemizygous and homozygous) zebrafish expressing the Odonthestes argentinensis growth hormone gene directed by the Cyprinus carpio beta-actin promoter. Higher growth parameters were observed in the hemizygous group. The homozygous group possessed higher oxygen consumption and reactive oxygen species production. Growth hormone transgenesis causes a decrease in glutamate-cysteine ligase catalytic subunit expression, an enzyme responsible for glutathione synthesis. Although the lipid hydroperoxide content was similar between groups, we demonstrate that growth hormone overexpression has the potential to generate oxidative stress in fishes.