PUBLICATION
Ethanol alters acetylcholinesterase activity and gene expression in zebrafish brain
- Authors
- Rico, E.P., Rosemberg, D.B., Dias, R.D., Bogo, M.R., and Bonan, C.D.
- ID
- ZDB-PUB-071001-3
- Date
- 2007
- Source
- Toxicology letters 174(1-3): 25-30 (Journal)
- Registered Authors
- Bonan, Carla Denise
- Keywords
- Ethanol, Acetaldehyde, Acetylcholinesterase, Zebrafish, Alcoholism
- MeSH Terms
-
- Acetaldehyde/toxicity
- Acetates/toxicity
- Acetylcholinesterase/genetics
- Acetylcholinesterase/metabolism*
- Animals
- Brain/drug effects*
- Brain/enzymology
- Central Nervous System Depressants/toxicity*
- Ethanol/toxicity*
- Gene Expression Regulation, Enzymologic/drug effects
- RNA, Messenger/metabolism
- Zebrafish
- PubMed
- 17888594 Full text @ Toxicol. Lett.
- CTD
- 17888594
Citation
Rico, E.P., Rosemberg, D.B., Dias, R.D., Bogo, M.R., and Bonan, C.D. (2007) Ethanol alters acetylcholinesterase activity and gene expression in zebrafish brain. Toxicology letters. 174(1-3):25-30.
Abstract
Alcohol abuse is a health problem throughout the world and alcohol consumption is linked to the occurrence of several pathological conditions. Acute ethanol administration exerts a variety of actions on the central nervous system (CNS). Zebrafish has been used as an attractive model system to investigate behavioral and neurochemical changes promoted by alcohol intoxication. Here we investigated the in vitro and in vivo effects promoted by ethanol and its metabolites on zebrafish brain acetylcholinesterase (AChE). There was a significant increase of AChE (33%) activity after acute 1% ethanol exposure. However, ethanol in vitro did not alter AChE activity. Acetaldehyde, the first metabolite of alcohol metabolism, promoted a dose-dependent decrease (15%, 27.5% and 46.5%) at 0.25%, 0.5% and 1%, respectively. Acetate, a product of acetaldehyde degradation, did not change AChE activity. Furthermore, the acute ethanol exposure was able to inhibit AChE transcripts at 0.5% and 1%. These findings suggest that the alterations on zebrafish AChE could reveal molecular mechanisms related to cholinergic signaling in alcoholism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping