PUBLICATION
Transient receptor potential channel M5 and phospholipaseC-beta2 colocalizing in zebrafish taste receptor cells
- Authors
- Yoshida, Y., Saitoh, K., Aihara, Y., Okada, S., Misaka, T., and Abe, K.
- ID
- ZDB-PUB-070924-7
- Date
- 2007
- Source
- Neuroreport 18(15): 1517-1520 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Chemoreceptor Cells/metabolism*
- DNA/genetics
- Databases, Genetic
- Green Fluorescent Proteins/genetics
- In Situ Hybridization
- Phospholipase C beta/genetics
- Phospholipase C beta/metabolism*
- Receptors, Cell Surface
- TRPM Cation Channels/genetics
- TRPM Cation Channels/metabolism*
- Taste Buds/metabolism*
- Zebrafish/physiology*
- PubMed
- 17885593 Full text @ Neuroreport
Citation
Yoshida, Y., Saitoh, K., Aihara, Y., Okada, S., Misaka, T., and Abe, K. (2007) Transient receptor potential channel M5 and phospholipaseC-beta2 colocalizing in zebrafish taste receptor cells. Neuroreport. 18(15):1517-1520.
Abstract
In mammals, transient receptor potential (TRP) channel M5 (TRPM5) is coexpressed with phospholipaseC-beta2 (PLC-beta2) in the taste receptor cells, and both PLC-beta2 and TRPM5 are essential elements in the signal transduction of sweet, bitter and umami stimuli. In this study, we identified the zebrafish homologue of TRPM5 (zfTRPM5) and examined its expression in the gustatory system by in-situ hybridization. Using a transgenic zebrafish line that expressed green fluorescent protein under the control of the PLC-beta2 promoter, we showed that zfTRPM5 is expressed in green fluorescent protein-labeled cells of the taste buds. These results demonstrate that zfTRPM5 and PLC-beta2 colocalize in zebrafish taste receptor cells, suggesting their crucial roles in taste signaling via the fish taste receptors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping