ZFIN ID: ZDB-PUB-070907-26
Target Protectors Reveal Dampening and Balancing of Nodal Agonist and Antagonist by miR-430
Choi, W.Y., Giraldez, A.J., and Schier, A.F.
Date: 2007
Source: Science (New York, N.Y.)   318(5848): 271-274 (Journal)
Registered Authors: Choi, Wen-Yee, Giraldez, Antonio, Schier, Alexander
Keywords: none
MeSH Terms:
  • 3' Untranslated Regions
  • Animals
  • Embryo, Nonmammalian/physiology
  • Embryonic Development
  • Gene Expression Regulation
  • Left-Right Determination Factors
  • MicroRNAs/metabolism*
  • Mutation
  • Nodal Protein
  • Nodal Signaling Ligands
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism*
  • Transforming Growth Factor beta/agonists
  • Transforming Growth Factor beta/antagonists & inhibitors
  • Transforming Growth Factor beta/genetics*
  • Transforming Growth Factor beta/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
PubMed: 17761850 Full text @ Science
MicroRNAs repress hundreds of target mRNAs, but the physiological roles of specific miRNA-mRNA interactions remain largely elusive. We report that zebrafish miR-430 dampens and balances the expression of the TGFss Nodal agonist squint and the TGFss Nodal antagonist lefty. To disrupt the interaction of specific miRNA-mRNA pairs, we developed target protector morpholinos complementary to miRNA binding sites in target mRNAs. Protection of squint or lefty mRNAs from miR-430 resulted in enhanced or reduced Nodal signaling, respectively. Simultaneous protection of squint and lefty or absence of miR-430 caused an imbalance and reduction in Nodal signaling. These findings establish an approach to analyze the in vivo roles of specific miRNA-mRNA pairs and reveal a requirement for miRNAs in dampening and balancing agonist/antagonist pairs.