|ZFIN ID: ZDB-PUB-070907-26|
Target Protectors Reveal Dampening and Balancing of Nodal Agonist and Antagonist by miR-430
Choi, W.Y., Giraldez, A.J., and Schier, A.F.
|Source:||Science (New York, N.Y.) 318(5848): 271-274 (Journal)|
|Registered Authors:||Choi, Wen-Yee, Giraldez, Antonio, Schier, Alexander|
|PubMed:||17761850 Full text @ Science|
Choi, W.Y., Giraldez, A.J., and Schier, A.F. (2007) Target Protectors Reveal Dampening and Balancing of Nodal Agonist and Antagonist by miR-430. Science (New York, N.Y.). 318(5848):271-274.
ABSTRACTMicroRNAs repress hundreds of target mRNAs, but the physiological roles of specific miRNA-mRNA interactions remain largely elusive. We report that zebrafish miR-430 dampens and balances the expression of the TGFss Nodal agonist squint and the TGFss Nodal antagonist lefty. To disrupt the interaction of specific miRNA-mRNA pairs, we developed target protector morpholinos complementary to miRNA binding sites in target mRNAs. Protection of squint or lefty mRNAs from miR-430 resulted in enhanced or reduced Nodal signaling, respectively. Simultaneous protection of squint and lefty or absence of miR-430 caused an imbalance and reduction in Nodal signaling. These findings establish an approach to analyze the in vivo roles of specific miRNA-mRNA pairs and reveal a requirement for miRNAs in dampening and balancing agonist/antagonist pairs.