| ZFIN ID: ZDB-PUB-070907-26 |
Target Protectors Reveal Dampening and Balancing of Nodal Agonist and Antagonist by miR-430
Choi, W.Y., Giraldez, A.J., and Schier, A.F.
| Date: | 2007 |
|---|---|
| Source: | Science (New York, N.Y.) 318(5848): 271-274 (Journal) |
| Registered Authors: | Choi, Wen-Yee, Giraldez, Antonio, Schier, Alexander |
| Keywords: | none |
| MeSH Terms: |
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| PubMed: | 17761850 Full text @ Science |
Citation
Choi, W.Y., Giraldez, A.J., and Schier, A.F. (2007) Target Protectors Reveal Dampening and Balancing of Nodal Agonist and Antagonist by miR-430. Science (New York, N.Y.). 318(5848):271-274.
ABSTRACT
MicroRNAs repress hundreds of target mRNAs, but the physiological roles of specific miRNA-mRNA interactions remain largely elusive. We report that zebrafish miR-430 dampens and balances the expression of the TGFss Nodal agonist squint and the TGFss Nodal antagonist lefty. To disrupt the interaction of specific miRNA-mRNA pairs, we developed target protector morpholinos complementary to miRNA binding sites in target mRNAs. Protection of squint or lefty mRNAs from miR-430 resulted in enhanced or reduced Nodal signaling, respectively. Simultaneous protection of squint and lefty or absence of miR-430 caused an imbalance and reduction in Nodal signaling. These findings establish an approach to analyze the in vivo roles of specific miRNA-mRNA pairs and reveal a requirement for miRNAs in dampening and balancing agonist/antagonist pairs.
ADDITIONAL INFORMATION
- Genes / Markers (10)
- Morpholino (5)
- Expression and Phenotype Data
- Mutations and Transgenics (3)
- Fish (19)