PUBLICATION
Target Protectors Reveal Dampening and Balancing of Nodal Agonist and Antagonist by miR-430
- Authors
- Choi, W.Y., Giraldez, A.J., and Schier, A.F.
- ID
- ZDB-PUB-070907-26
- Date
- 2007
- Source
- Science (New York, N.Y.) 318(5848): 271-274 (Journal)
- Registered Authors
- Choi, Wen-Yee, Giraldez, Antonio, Schier, Alexander
- Keywords
- none
- MeSH Terms
-
- 3' Untranslated Regions
- Animals
- Embryo, Nonmammalian/physiology
- Embryonic Development
- Gene Expression Regulation
- Left-Right Determination Factors
- MicroRNAs/metabolism*
- Mutation
- Nodal Protein
- Nodal Signaling Ligands
- RNA, Messenger/genetics
- RNA, Messenger/metabolism*
- Transforming Growth Factor beta/agonists
- Transforming Growth Factor beta/antagonists & inhibitors
- Transforming Growth Factor beta/genetics*
- Transforming Growth Factor beta/metabolism*
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- PubMed
- 17761850 Full text @ Science
Citation
Choi, W.Y., Giraldez, A.J., and Schier, A.F. (2007) Target Protectors Reveal Dampening and Balancing of Nodal Agonist and Antagonist by miR-430. Science (New York, N.Y.). 318(5848):271-274.
Abstract
MicroRNAs repress hundreds of target mRNAs, but the physiological roles of specific miRNA-mRNA interactions remain largely elusive. We report that zebrafish miR-430 dampens and balances the expression of the TGFss Nodal agonist squint and the TGFss Nodal antagonist lefty. To disrupt the interaction of specific miRNA-mRNA pairs, we developed target protector morpholinos complementary to miRNA binding sites in target mRNAs. Protection of squint or lefty mRNAs from miR-430 resulted in enhanced or reduced Nodal signaling, respectively. Simultaneous protection of squint and lefty or absence of miR-430 caused an imbalance and reduction in Nodal signaling. These findings establish an approach to analyze the in vivo roles of specific miRNA-mRNA pairs and reveal a requirement for miRNAs in dampening and balancing agonist/antagonist pairs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping