PUBLICATION
The zebrafish erythropoietin: Functional identification and biochemical characterization
- Authors
- Chu, C.Y., Cheng, C.H., Chen, G.D., Chen, Y.C., Hung, C.C., Huang, K.Y., and Huang, C.J.
- ID
- ZDB-PUB-070827-6
- Date
- 2007
- Source
- FEBS letters 581(22): 4265-4271 (Journal)
- Registered Authors
- Keywords
- Erythropoietin, Zebrafish, Morpholino oligonucleotide, Glycoprotein, Secretion, Globin staining
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- COS Cells
- Chlorocebus aethiops
- Cloning, Molecular
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Erythroid Cells/metabolism
- Erythropoietin/chemistry
- Erythropoietin/genetics*
- Erythropoietin/metabolism*
- Gene Expression Profiling
- Gene Expression Regulation, Developmental
- Hemoglobins/biosynthesis
- Molecular Sequence Data
- Organ Specificity
- Protein Transport
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Sequence Alignment
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 17706649 Full text @ FEBS Lett.
Citation
Chu, C.Y., Cheng, C.H., Chen, G.D., Chen, Y.C., Hung, C.C., Huang, K.Y., and Huang, C.J. (2007) The zebrafish erythropoietin: Functional identification and biochemical characterization. FEBS letters. 581(22):4265-4271.
Abstract
In the present study, the zebrafish epo cDNA was cloned. The encoded protein displays 90%, 55% and 32% identity to the Epo from carp, fugu and human, respectively. Through RT-PCR, the expression of zepo mRNA was mainly in the heart and liver. In the COS-1 cell transfection experiments, the recombinant zEpo-HA protein was efficiently secreted into the culture medium as a glycoprotein and the carbohydrate moiety can be cleaved by the treatment of peptide-N-glycosidase F (PNGase F). Using the morpholino approach, we showed that zepo morphants displayed severe anemia leading to high mortality during development. Such an effect can be significantly rescued by zepo RNA. Furthermore, in the absence of functional zEpo, the expression of specific markers for adult globin genes, such as alphaA1- and betaA1-globin, but not the embryonic betae1-globin, was affected.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping