ZFIN ID: ZDB-PUB-070827-28
Light-controlled gene silencing in zebrafish embryos
Shestopalov, I.A., Sinha, S., and Chen, J.K.
Date: 2007
Source: Nature Chemical Biology   3(10): 650-651 (Journal)
Registered Authors: Chen, James K., Sinha, Surajit
Keywords: none
MeSH Terms:
  • Animals
  • Body Patterning
  • Embryo, Nonmammalian/physiology
  • Embryo, Nonmammalian/radiation effects*
  • Gene Expression Regulation, Developmental/physiology
  • Gene Expression Regulation, Developmental/radiation effects*
  • Gene Silencing/physiology
  • Gene Silencing/radiation effects*
  • Glycoproteins/biosynthesis
  • Glycoproteins/chemistry
  • Glycoproteins/genetics
  • Light*
  • Peptide Chain Initiation, Translational
  • RNA Splicing/drug effects*
  • RNA Splicing/physiology
  • Ultraviolet Rays
  • Zebrafish
PubMed: 17717538 Full text @ Nat. Chem. Biol.
Functional genomic studies in zebrafish frequently use synthetic oligonucleotides called morpholinos that block RNA splicing or translation. However, the constitutive activity of these reagents limits their experimental utility. We report here the synthesis of a photoactivatable morpholino targeting the no tail (ntl) gene. This caged reagent permits spatiotemporal gene regulation in vivo and the photochemical generation of functionally mosaic organisms.