PUBLICATION

Duplication and divergence of fgf8 functions in teleost development and evolution

Authors
Jovelin, R., He, X., Amores, A., Yan, Y.L., Shi, R., Qin, B., Roe, B., Cresko, W.A., and Postlethwait, J.H.
ID
ZDB-PUB-070827-11
Date
2007
Source
Journal of experimental zoology. Part B, Molecular and developmental evolution   308B(6): 730-743 (Journal)
Registered Authors
Amores, Angel, Cresko, William, Postlethwait, John H., Yan, Yi-Lin
Keywords
none
MeSH Terms
  • Animals
  • Evolution, Molecular*
  • Fibroblast Growth Factors/genetics*
  • Fishes/embryology*
  • Fishes/genetics*
  • Fishes/physiology
  • Gene Duplication*
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Genes, Duplicate/genetics*
  • Phylogeny
  • Signal Transduction
PubMed
17708537 Full text @ J. Exp. Zool. B Mol. Dev. Evol.
Abstract
Fibroblast growth factors play critical roles in many aspects of embryo patterning that are conserved across broad phylogenetic distances. To help understand the evolution of fibroblast growth factor functions, we identified members of the Fgf8/17/18-subfamily in the three-spine stickleback Gasterosteus aculeatus, and investigated their evolutionary relationships and expression patterns. We found that fgf17b is the ortholog of tetrapod Fgf17, whereas the teleost genes called fgf8 and fgf17a are duplicates of the tetrapod gene Fgf8, and thus should be called fgf8a and fgf8b. Phylogenetic analysis supports the view that the Fgf8/17/18-subfamily expanded during the ray-fin fish genome duplication. In situ hybridization experiments showed that stickleback fgf8 duplicates exhibited common and unique expression patterns, indicating that tissue specialization followed the gene duplication event. Moreover, direct comparison of stickleback and zebrafish embryonic expression patterns of fgf8 co-orthologs suggested lineage-specific independent subfunction partitioning and the acquisition or the loss of ortholog functions. In tetrapods, Fgf8 plays an important role in the apical ectodermal ridge of the developing pectoral appendage. Surprisingly, differences in the expression of fgf8a in the apical ectodermal ridge of the pectoral fin bud in zebrafish and stickleback, coupled with the role of fgf16 and fgf24 in teleost pectoral appendage show that different Fgf genes may play similar roles in limb development in various vertebrates.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping