PUBLICATION

Nodal activity around Kupffer's vesicle depends on the T-box transcription factors notail and spadetail and on notch signaling

Authors
Gourronc, F., Ahmad, N., Nedza, N., Eggleston, T., and Rebagliati, M.
ID
ZDB-PUB-070806-16
Date
2007
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   236(8): 2131-2146 (Journal)
Registered Authors
Ahmad, Nadira, Rebagliati, Michael
Keywords
zebrafish, asymmetry, brachyury, notail, spadetail, southpaw, nodal, notch
MeSH Terms
  • Zebrafish
  • Embryo, Nonmammalian
  • Nodal Protein
  • Fetal Proteins/physiology*
  • Drosophila Proteins/physiology*
  • T-Box Domain Proteins/physiology*
  • Zebrafish Proteins/physiology*
  • Transforming Growth Factor beta/physiology*
  • Receptors, Notch/physiology*
  • Animals
  • Signal Transduction/physiology*
  • Body Patterning*
(all 12)
PubMed
17654709 Full text @ Dev. Dyn.
Abstract
The node, or its zebrafish equivalent, Kupffers Vesicle (KV), is thought to generate laterality cues through cilia-dependent signaling. An interaction between Nodal ligands and Nodal antagonists around the node/KV is also required. Here we investigate whether loss of Brachyury/Notail or Tbx16/Spadetail disrupts the balance of Nodal ligands (Southpaw) and antagonists (Charon) around Kupffers Vesicle. Reduction of Spadetail or Notail disrupts expression of southpaw in the perinodal domains flanking Kupffers Vesicle. Similar to what was published for Notail, we find Spadetail is also required for expression of charon. We present evidence for the model that Notail has a direct role in regulating the charon promoter. In particular, a flanking genomic region with putative Notail binding sites can drive KV expression of a reporter in a Notail-dependent fashion. This region also contains motifs for CSL/RBP-J/Su(H). Consistent with this, we find charon expression is strongly Notch-dependent whereas perinodal southpaw expression is not.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b160
    Indel
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    dnah9MO1-dnah9MRPHLNO
    pdia6MO2-pdia6MRPHLNO
    spawMO1-spawMRPHLNO
    spawMO2-spawMRPHLNO
    tbx16MO1-tbx16MRPHLNO
    tbx16MO2-tbx16MRPHLNO
    tbxtaMO1-tbxtaMRPHLNO
    1 - 7 of 7
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    Fish
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available