PUBLICATION
Nodal activity around Kupffer's vesicle depends on the T-box transcription factors notail and spadetail and on notch signaling
- Authors
- Gourronc, F., Ahmad, N., Nedza, N., Eggleston, T., and Rebagliati, M.
- ID
- ZDB-PUB-070806-16
- Date
- 2007
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 236(8): 2131-2146 (Journal)
- Registered Authors
- Ahmad, Nadira, Rebagliati, Michael
- Keywords
- zebrafish, asymmetry, brachyury, notail, spadetail, southpaw, nodal, notch
- MeSH Terms
-
- Fetal Proteins/physiology*
- T-Box Domain Proteins/physiology*
- Zebrafish Proteins/physiology*
- Drosophila Proteins/physiology*
- Nodal Protein
- Embryo, Nonmammalian
- Zebrafish
- Receptors, Notch/physiology*
- Animals
- Transforming Growth Factor beta/physiology*
- Signal Transduction/physiology*
- Body Patterning*
- PubMed
- 17654709 Full text @ Dev. Dyn.
Citation
Gourronc, F., Ahmad, N., Nedza, N., Eggleston, T., and Rebagliati, M. (2007) Nodal activity around Kupffer's vesicle depends on the T-box transcription factors notail and spadetail and on notch signaling. Developmental Dynamics : an official publication of the American Association of Anatomists. 236(8):2131-2146.
Abstract
The node, or its zebrafish equivalent, Kupffers Vesicle (KV), is thought to generate laterality cues through cilia-dependent signaling. An interaction between Nodal ligands and Nodal antagonists around the node/KV is also required. Here we investigate whether loss of Brachyury/Notail or Tbx16/Spadetail disrupts the balance of Nodal ligands (Southpaw) and antagonists (Charon) around Kupffers Vesicle. Reduction of Spadetail or Notail disrupts expression of southpaw in the perinodal domains flanking Kupffers Vesicle. Similar to what was published for Notail, we find Spadetail is also required for expression of charon. We present evidence for the model that Notail has a direct role in regulating the charon promoter. In particular, a flanking genomic region with putative Notail binding sites can drive KV expression of a reporter in a Notail-dependent fashion. This region also contains motifs for CSL/RBP-J/Su(H). Consistent with this, we find charon expression is strongly Notch-dependent whereas perinodal southpaw expression is not.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping