PUBLICATION
Laser-scanning velocimetry: A confocal microscopy method for quantitative measurement of cardiovascular performance in zebrafish embryos and larvae
- Authors
- Malone, M.H., Sciaky, N., Stalheim, L., Hahn, K.M., Linney, E., and Johnson, G.L.
- ID
- ZDB-PUB-070726-6
- Date
- 2007
- Source
- BMC Biotechnology 7(1): 40 (Journal)
- Registered Authors
- Linney, Elwood
- Keywords
- none
- MeSH Terms
-
- RNA, Messenger/administration & dosage
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Aorta/drug effects
- Aorta/embryology
- Aorta/physiology
- Aminobenzoates/pharmacology
- Genetic Complementation Test
- Microscopy, Confocal/methods*
- Animals
- Microfilament Proteins/genetics
- Microfilament Proteins/metabolism
- Microfilament Proteins/physiology
- Reproducibility of Results
- Heart/embryology
- Heart/physiology*
- Blood Flow Velocity/drug effects
- Myocardium/cytology
- Myocardium/metabolism
- Zebrafish/embryology
- Zebrafish/physiology*
- Microinjections
- Mutation
- Larva/cytology
- Larva/genetics
- Larva/physiology
- Gene Expression Regulation, Developmental
- Blotting, Western
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/physiology
- Morpholines/pharmacology
- PubMed
- 17623073 Full text @ BMC Biotechnol.
Citation
Malone, M.H., Sciaky, N., Stalheim, L., Hahn, K.M., Linney, E., and Johnson, G.L. (2007) Laser-scanning velocimetry: A confocal microscopy method for quantitative measurement of cardiovascular performance in zebrafish embryos and larvae. BMC Biotechnology. 7(1):40.
Abstract
BACKGROUND: The zebrafish Danio rerio is an important model system for drug discovery and to study cardiovascular development. Using a laser-scanning confocal microscope, we have developed a non-invasive method of measuring cardiac performance in zebrafish embryos and larvae that obtains cardiovascular parameters similar to those obtained using Doppler echocardiography in mammals. A laser scan line placed parallel to the path of blood in the dorsal aorta measures blood cell velocity, from which cardiac output and indices of vascular resistance and contractility are calculated. RESULTS: This technique, called laser-scanning velocimetry, was used to quantify the effects of pharmacological, developmental, and genetic modifiers of cardiac function. Laser-scanning velocimetry was applied to analyze the cardiovascular effects of morpholino knockdown of osmosensing scaffold for MEKK3 (OSM), which when mutated causes the human vascular disease cerebral cavernous malformations. OSM-deficient embryos had a constricted aortic arch and markedly increased peak cell velocity, a characteristic indicator of aortic stenosis. CONCLUSIONS: These data validate laser-scanning velocimetry as a quantitative tool to measure cardiovascular performance for pharmacological and genetic analysis in zebrafish, which requires no specialized equipment other than a laser-scanning confocal microscope.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping