PUBLICATION
Novel zebrafish caspase-3 substrates
- Authors
- Valencia, C.A., Bailey, C., and Liu, R.
- ID
- ZDB-PUB-070726-32
- Date
- 2007
- Source
- Biochemical and Biophysical Research Communications 361(2): 311-316 (Journal)
- Registered Authors
- Keywords
- Danio rerio, Zebrafish caspase-3, Natural caspase substrates, Caspase cleavage site, Comparison of caspases from different species
- MeSH Terms
-
- Animals
- Binding Sites
- Caspase 3/metabolism*
- Endopeptidases/metabolism
- Humans
- Peptide Fragments/metabolism
- Protein Processing, Post-Translational
- Sequence Homology, Amino Acid
- Species Specificity
- Substrate Specificity
- Zebrafish/metabolism*
- Zebrafish Proteins/metabolism
- PubMed
- 17643392 Full text @ Biochem. Biophys. Res. Commun.
Citation
Valencia, C.A., Bailey, C., and Liu, R. (2007) Novel zebrafish caspase-3 substrates. Biochemical and Biophysical Research Communications. 361(2):311-316.
Abstract
The zebrafish model has been widely used to investigate numerous signaling pathways in vertebrates, including programmed cell death. Although several zebrafish proteins homologous to mammalian caspases have been identified, our understanding of these zebrafish caspases is still limited. Recently, we identified a large number of natural caspase-3 substrates from the human proteome by using the mRNA-display selection method. Through comparative analysis, we found that the cleavage sites on some of these novel human caspase-3 substrates are highly conserved in their zebrafish orthologs. We report here the identification and characterization of 14 natural zebrafish caspase-3 substrates that have not yet been previously studied. The specific cleavage of these zebrafish proteins was compared with caspases from different species, and the protein fragments that contain the putative cleavage sites were mapped. The work described here could facilitate our understanding of the downstream signaling pathways that are mediated by caspase-3 in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping