Ultrasound biomicroscopy permits in vivo characterization of zebrafish liver tumors
- Goessling, W., North, T.E., and Zon, L.I.
- Nature Methods 4(7): 551-553 (Journal)
- Registered Authors
- Goessling, Wolfram, North, Trista, Zon, Leonard I.
- MeSH Terms
- Disease Models, Animal*
- Liver Neoplasms, Experimental/pathology
- Microscopy, Acoustic/methods*
- 17572681 Full text @ Nat. Methods
Goessling, W., North, T.E., and Zon, L.I. (2007) Ultrasound biomicroscopy permits in vivo characterization of zebrafish liver tumors. Nature Methods. 4(7):551-553.
Zebrafish are a valuable vertebrate model to study carcinogenesis, but noninvasive imaging is challenging because adult fish are not transparent. Here we show that tumors could be readily detected in vivo using high-resolution microscopic ultrasound in zebrafish. We successfully obtained tissue perfusion calculations and cellular aspirates, and analyzed tumor progression and response to treatment. Ultrasound biomicroscopy allows longitudinal studies of tumor development and real-time assessment of therapeutic effects in zebrafish.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes