PUBLICATION

MiRNA expression analysis during normal zebrafish development and following inhibition of the Hedgehog and Notch signaling pathways

Authors
Thatcher, E.J., Flynt, A.S., Li, N., Patton, J.R., and Patton, J.G.
ID
ZDB-PUB-070629-28
Date
2007
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   236(8): 2172-2180 (Journal)
Registered Authors
Li, Nan
Keywords
miRNA, microarray, Notch, Hedgehog, cyclopamine, DAPT
MeSH Terms
  • Animals
  • Embryonic Development/genetics
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental*
  • Hedgehog Proteins/antagonists & inhibitors*
  • MicroRNAs/genetics*
  • RNA, Messenger/analysis
  • Receptors, Notch/antagonists & inhibitors*
  • Signal Transduction*
  • Zebrafish
PubMed
17584857 Full text @ Dev. Dyn.
Abstract
microRNAs (miRNAs) are small ( approximately 22 nucleotide) non-coding RNAs that regulate gene expression at the post-transcriptional level, typically by inhibiting translation. The genes encoding these small RNAs are estimated to comprise approximately 2-3% of animal genomes yet potentially regulate a majority of protein-coding genes including those involved in cell specification and development. A key remaining question is to identify target mRNAs regulated by microRNAs. As a means to identify potential targets, we designed a sensitive microarray to analyze global miRNA expression patterns at twelve developmental stages in zebrafish. Further, we conducted arrays on zebrafish embryos treated with small molecule inhibitors of the Hedgehog and Notch signaling pathways to enable identification of differentially expressed miRNAs that target genes controlling key developmental pathways during early embryogenesis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping