PUBLICATION
High Transcript Level of Fatty Acid-Binding Protein 11 But Not of Very Low-Density Lipoprotein Receptor Is Correlated to Ovarian Follicle Atresia in a Teleost Fish (Solea senegalensis)
- Authors
- Agulleiro, M.J., Andre, M., Morais, S., Cerda, J., and Babin, P.J.
- ID
- ZDB-PUB-070625-15
- Date
- 2007
- Source
- Biology of reproduction 77(3): 504-516 (Journal)
- Registered Authors
- Babin, Patrick J., Cerdà, Joan
- Keywords
- Ovary, Follicular development, Gametogenesis, Gene regulation, Oocyte development
- MeSH Terms
-
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Fatty Acid-Binding Proteins/biosynthesis*
- Fatty Acid-Binding Proteins/genetics
- Molecular Sequence Data
- Phylogeny
- In Situ Hybridization
- Base Sequence
- Sequence Alignment
- Follicular Atresia/metabolism
- Follicular Atresia/physiology*
- Ovarian Follicle/metabolism
- Ovarian Follicle/physiology*
- Blotting, Northern
- Receptors, LDL/biosynthesis*
- Receptors, LDL/genetics
- Sequence Analysis, DNA
- Reverse Transcriptase Polymerase Chain Reaction
- Amino Acid Sequence
- Transcription, Genetic
- Female
- Vitellogenesis/genetics
- Vitellogenesis/physiology
- Flatfishes/genetics*
- Flatfishes/metabolism
- Animals
- PubMed
- 17554079 Full text @ Biol. Reprod.
Citation
Agulleiro, M.J., Andre, M., Morais, S., Cerda, J., and Babin, P.J. (2007) High Transcript Level of Fatty Acid-Binding Protein 11 But Not of Very Low-Density Lipoprotein Receptor Is Correlated to Ovarian Follicle Atresia in a Teleost Fish (Solea senegalensis). Biology of reproduction. 77(3):504-516.
Abstract
Transcripts encoding a fatty acid-binding protein (FABP), Fabp11, and two isoforms of very low-density lipoprotein receptor (Vldlr) (vitellogenin receptor) were characterized from the ovary of Senegalese sole (Solea senegalensis). Phylogenetic analyses of vertebrate FABPs demonstrated that Senegalese sole Fabp11, as zebrafish (Danio rerio) homologous sequences, was part of a newly defined teleost fish FABP subfamily which was a sister clade of tetrapod FABP4/FABP5/FABP8/FABP9. Reverse transcriptase polymerase chain reaction (RT-PCR) revealed high levels of vldlr transcript splicing variants in the ovaries and, to a lesser extent, in somatic tissues, while fabp11 was highly expressed in the ovaries, liver, and adipose tissue. In situ hybridization analysis showed vldlr and fabp11 mRNAs in previtellogenic oocytes, while no hybridization signals were detected in the larger vitellogenic oocytes. fabp11 transcript expression was strongly up-regulated in somatic cells surrounding atretic follicles. Real-time quantitative RT-PCR demonstrated that ovarian transcript levels of vldlr and fabp11 had a significant positive correlation with the percentage of follicles in previtellogenesis and atresia, respectively. These results suggest that the expression level of vldlr transcripts may be used as a precocious functional marker to quantify the number of oocytes recruited for vitellogenesis and that fabp11 mRNA may be a very useful molecular marker for determining cellular events and environmental factors that regulate follicular atresia in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping